| Title: | Ultra-Sensitive Isopropanol Biochemical Gas Sensor (Bio-Sniffer) for Monitoring of Human Volatiles |
| Author(s): | Chien PJ; Suzuki T; Ye M; Toma K; Arakawa T; Iwasaki Y; Mitsubayashi K; |
| Address: | "Institute of Chemistry, Academia Sinica, No. 128, Sec. 2, Academia Rd., Nankang, Taipei 115, Taiwan. Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-Ku, Tokyo 113-8549, Japan. Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan. Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamate-Cho, Suita-Shi, Osaka 564-0836, Japan" |
| ISSN/ISBN: | 1424-8220 (Electronic) 1424-8220 (Linking) |
| Abstract: | "Our groups have previously developed a biochemical gas sensor to measure isopropanol (IPA) in exhaled air and have applied it for breath IPA investigation in healthy subjects and diabetes patients. In this study, the original bio-sniffer was modified with a series of components that improved the limit of detection (LOD). First, the modified IPA bio-sniffer used a C8855-type photomultiplier tube (PMT) that performed well in the photon sensitivity at the peak wavelength of nicotinamide adenine dinucleotide (NADH) fluorescence. Second, the multi-core bifurcated optical fiber, which incorporated 36 fibers to replace the previous dual-core type, enhanced the fluorescence collection. Third, the optical fiber probe was reinforced for greater width, and the flow-cell was redesigned to increase the area of the enzyme-immobilized membrane in contact with the air sample. These modifications lowered the detection limit to 0.5 ppb, a significant increase over the previous 1.0 ppb. Moreover, the modified bio-sniffer successfully analyzed the IPA concentration in exhaled air from a volunteer, which confirmed its capability for real-world sample detection. The modified bio-sniffer is more applicable to breath measurement and the detection of other extremely-low-concentration samples" |
| Keywords: | *2-Propanol *Biosensing Techniques *Breath Tests Exhalation Humans Optical Fibers Nadh biosensor breath isopropanol gas sensor secondary alcohol dehydrogenase volatile organic compounds; |
| Notes: | "MedlineChien, Po-Jen Suzuki, Takuma Ye, Ming Toma, Koji Arakawa, Takahiro Iwasaki, Yasuhiko Mitsubayashi, Kohji eng JP26280053/Japan Society for the Promotion of Science/ JP17H01759/Japan Society for the Promotion of Science/ Switzerland 2020/12/03 Sensors (Basel). 2020 Nov 29; 20(23):6827. doi: 10.3390/s20236827" |
