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J Chromatogr A

Title:		"Quantification of 4-hydroxy-2,5-dimethyl-3-furanone in fruit samples using solid phase microextraction coupled with gas chromatography-mass spectrometry"
Author(s):		Chen Y; Sidisky LM;
Address:		"Supelco, 595 North Harrison Road, Bellefonte, PA 16823, USA. yong.chen@sial.com"
Journal Title:		J Chromatogr A
Year:		2011
Volume:		20110806
Issue:		38
Page Number:		6817 - 6822
DOI:		10.1016/j.chroma.2011.07.103
ISSN/ISBN:		1873-3778 (Electronic) 0021-9673 (Linking)
Abstract:		"Furaneol is an important aroma compound. It is very difficult to extract furaneol from food matrices and separate it on a gas chromatography column due to its high polarity and instability. A new quantitative method was developed to quantify furaneol in aqueous samples by the use of derivatization/solid phase microextraction (SPME) coupled with gas chromatography/mass spectrometry (GC/MS). The derivatization was carried out by reacting pentafluorobenzyl bromide with furaneol in basic solutions at elevated temperatures. The derivative was stable and less polar so that SPME-GC/MS could be applied for extraction, separation and detection. The automated analytical method had a limit of detection (LOD) of 0.5 ng mL(-1), a limit of quantification (LOQ) of 2 ng mL(-1), a repeatability of 9.5%, and a linear range from 2 to 500 ng mL(-1). The method was applied to analyze fruit samples. And it was found that the concentrations of furaneol in tomato ranged from 95 to 173 mug kg(-1), in strawberries ranged from 1663 to 4852 mug kg(-1). The results were verified with a LC procedure. To facilitate analytical method development, some physico-chemical parameters for furaneol were determined in this work. Its solubility in water was determined as 0.315 g mL(-1) (25 degrees C). Its LogD in water and LogP in 0.1 M phosphate buffer were -0.133 and 0.95 (20 degrees C), respectively. Its pKa was 8.56 (20 degrees C)"
Keywords:		Food Analysis Fruit/*chemistry Furans/*analysis/chemistry/*isolation & purification Gas Chromatography-Mass Spectrometry/*methods Solid Phase Microextraction/*methods Volatile Organic Compounds/*analysis/*isolation & purification;
Notes:		"MedlineChen, Yong Sidisky, Leonard M eng Evaluation Study Netherlands 2011/08/23 J Chromatogr A. 2011 Sep 23; 1218(38):6817-22. doi: 10.1016/j.chroma.2011.07.103. Epub 2011 Aug 6"