| Title: | 1-MCP treatment affects peach fruit aroma metabolism as revealed by transcriptomics and metabolite analyses |
| Author(s): | Cai H; Han S; Jiang L; Yu M; Ma R; Yu Z; |
| Address: | "College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Institute of Pomology, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Jiangsu 210095, PR China. College of Food Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China. Electronic address: yuzhifang@njau.edu.cn" |
| DOI: | 10.1016/j.foodres.2019.01.026 |
| ISSN/ISBN: | 1873-7145 (Electronic) 0963-9969 (Linking) |
| Abstract: | "1-methylcyclopropene (1-MCP) negatively affects peach aroma but the underlying molecular basis remains elusive. In this study, transcriptomics and metabolite analyses were carried out to investigate the regulatory mechanisms of 1-MCP on peach aroma from different standpoints: fatty acid (FA) metabolism, ethylene signal transduction and lipoxygenase/beta-oxidation pathway during 20?ª+ degrees C storage. Results indicate that 1-MCP significantly postponed the ethylene climacteric peak appearance and reduced ethylene production through down-regulation of related biosynthesis and signal transduction genes including PpaSAMS1/2, PpaACS1/2, PpaACO1 together with PpaETR1/2, PpaERS1, PpaEIN4 and PpaCTR1. Decrease in the levels of FAs and PpaFADs was observed in treated fruit, except oleic acid and PpaFAD4/5, before day 5 of storage. In addition, 1-MCP-treated fruit also possessed higher levels of C6 aldehydes and alcohols and delayed the formation of volatile compounds characteristic of peach-like aroma by upregulation of PpaLOX1/2/3 and PpaHPL1 expression and down-regulation of PpaLOX5 expression. Our findings suggest that inhibition of peach-like volatiles and promotion of green-note volatiles by 1-MCP were associated with ethylene production and modulation of FA levels through transcriptional regulation" |
| Keywords: | "Cyclopropanes/*analysis/*pharmacology Ethylenes/analysis Fruit/*chemistry Gene Expression Regulation, Plant Odorants/*analysis Plant Growth Regulators/*analysis/*pharmacology Plant Proteins/genetics/metabolism Prunus persica/*chemistry/genetics Transcript;" |
| Notes: | "MedlineCai, Hongfang Han, Shuai Jiang, Li Yu, Mingliang Ma, Ruijuan Yu, Zhifang eng Research Support, Non-U.S. Gov't Canada 2019/06/24 Food Res Int. 2019 Aug; 122:573-584. doi: 10.1016/j.foodres.2019.01.026. Epub 2019 Jan 11" |
