« Previous AbstractInvestigation of Ground-Level Ozone and High-Pollution Episodes in a Megacity of Eastern China    Next Abstract"Observation of the fine structure of antennal sensilla of the stink bug, Eocanthecona furcellata (Hemiptera: Pentatomidae)" »

Insects

Title:		"Expression Profile and Ligand Screening of a Putative Odorant-Binding Protein, AcerOBP6, from the Asian Honeybee"
Author(s):		Zhao H; Peng Z; Huang L; Zhao S; Liu M;
Address:		"College of Life Science, Shanxi Agricultural University, Jinzhong 030801, China"
Journal Title:		Insects
Year:		2021
Volume:		20211020
Issue:		11
Page Number:		-
DOI:		10.3390/insects12110955
ISSN/ISBN:		2075-4450 (Print) 2075-4450 (Electronic) 2075-4450 (Linking)
Abstract:		"Olfaction is essential in some behaviors of honeybee, such as nursing, foraging, attracting a mate, social communication, and kin recognition. OBPs (odorant binding proteins) play a key role in the first step of olfactory perception. Here, we focused on a classic OBP with a PBP-GOBP domain from the Asian honeybee, Apis cerana cerana. Beyond that, the mRNA expression profiles and the binding affinity of AcerOBP6 were researched. According to qRT-PCR analysis, AcerOBP6 transcripts were mainly expressed in the antennae of forager bees. In addition, we found that the expression level of AcerOBP6 was higher than that of AmelOBP6. The fluorescence competitive binding assay indicated that the AcerOBP6 protein had binding affinity with most of the tested odors, including queen pheromone, worker pheromone, and floral volatiles, among which the strongest one was linolenic acid (with a Ki value of 1.67). However, AcerOBP6 was not sensitive to the brood pheromones. A further study based on EAG assay revealed that the antennae had the strongest response to 2-heptanone. The EAG recording values of the selected ligands were all reduced after AcerOBP6 was silenced, with 8 of 14 declining significantly (p < 0.01) given that these odors could specifically bind to AcerOBP6. As revealed in our current study, AcerOBP6 might be a crucial protein involved in olfactory recognition for foraging. Overall, the research provides a foundation for exploring the olfactory mechanism of A. cerana cerana"
Keywords:		Eag fluorescence binding assay honeybee odorant binding protein;
Notes:		"PubMed-not-MEDLINEZhao, Huiting Peng, Zhu Huang, Li Zhao, Shuguo Liu, Miaomiao eng 31502021/National Natural Science Foundation of China/ CARS-44-KXJ23/the China Agriculture Research System of MOF and MARA/ Switzerland 2021/11/26 Insects. 2021 Oct 20; 12(11):955. doi: 10.3390/insects12110955"