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Insect Biochem Mol Biol

Title:		Odorant receptors from Culex quinquefasciatus and Aedes aegypti sensitive to floral compounds
Author(s):		Zeng F; Xu P; Leal WS;
Address:		"Department of Molecular and Cellular Biology, University of California, Davis, CA, 95616, USA. Department of Molecular and Cellular Biology, University of California, Davis, CA, 95616, USA. Electronic address: wsleal@ucdavis.edu"
Journal Title:		Insect Biochem Mol Biol
Year:		2019
Volume:		20190820
Issue:
Page Number:		103213 -
DOI:		10.1016/j.ibmb.2019.103213
ISSN/ISBN:		1879-0240 (Electronic) 0965-1748 (Print) 0965-1748 (Linking)
Abstract:		"Mosquitoes rely heavily on the olfactory system to find a host for a bloodmeal, plants for a source of energy and suitable sites for oviposition. Here, we examined a cluster of eight odorant receptors (ORs), which includes one OR, CquiOR1, previously identified to be sensitive to plant-derived compounds. We cloned 5 ORs from Culex quinquefasciatus and two ORs from Aedes aegypti, ie, CquiOR2, CquiOR4, CquiOR5, CquiOR84, CquiOR85, AaegOR14, and AaegOR15 and then deorphanized these receptors using the Xenopus oocyte recording system and a large panel of odorants. 2-Phenylethanol, phenethyl formate, and phenethyl propionate were the best ligands for CquiOR4 somewhat resembling the profile of AaegOR15, which gave the strongest responses to phenethyl propionate, phenethyl formate, and acetophenone. In contrast, the best ligands for CquiOR5 were linalool, PMD, and linalool oxide. CquiOR4 was predominantly expressed in antennae of nonblood fed female mosquitoes, with transcript levels significantly reduced after a blood meal. 2-Phenylethanol showed repellency activity comparable to that of DEET at 1%. RNAi experiments suggest that at least in part 2-phenylethanol-elicited repellency is mediated by CquiOR4 activation"
Keywords:		"Aedes/genetics/*physiology Animals Chemotaxis Culex/genetics/*physiology Female Flowers/*chemistry Insect Proteins/*genetics/metabolism Insect Repellents/pharmacology Odorants/*analysis Receptors, Odorant/*genetics/metabolism Species Specificity 2-Phenyle;"
Notes:		"MedlineZeng, Fangfang Xu, Pingxi Leal, Walter S eng R01 AI095514/AI/NIAID NIH HHS/ Research Support, N.I.H., Extramural England 2019/08/24 Insect Biochem Mol Biol. 2019 Oct; 113:103213. doi: 10.1016/j.ibmb.2019.103213. Epub 2019 Aug 20"