| Title: | Inhibition of enteric methanogenesis in dairy cows induces changes in plasma metabolome highlighting metabolic shifts and potential markers of emission |
| Author(s): | Yanibada B; Hohenester U; Petera M; Canlet C; Durand S; Jourdan F; Boccard J; Martin C; Eugene M; Morgavi DP; Boudra H; |
| Address: | "Universite Clermont Auvergne, INRAE, VetAgro Sup, UMR Herbivores, Saint-Genes-Champanelle, Clermont-Ferrand, France. Universite Clermont Auvergne, INRAE, UNH, Plateforme d'Exploration du Metabolisme, MetaboHUB Clermont, 63000, Clermont-Ferrand, France. Toxalim, Research Centre in Food Toxicology, Universite de Toulouse, INRAE, ENVT, INP-Purpan, UPS, 31027, Toulouse, France. Axiom Platform, MetaToul-MetaboHUB, National Infrastructure for Metabolomics and Fluxomics, 31027, Toulouse, France. Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, Geneva, Switzerland. Universite Clermont Auvergne, INRAE, VetAgro Sup, UMR Herbivores, Saint-Genes-Champanelle, Clermont-Ferrand, France. diego.morgavi@inrae.fr. Universite Clermont Auvergne, INRAE, VetAgro Sup, UMR Herbivores, Saint-Genes-Champanelle, Clermont-Ferrand, France. abdelhamid.boudra@inrae.fr" |
| DOI: | 10.1038/s41598-020-72145-w |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "There is scarce information on whether inhibition of rumen methanogenesis induces metabolic changes on the host ruminant. Understanding these possible changes is important for the acceptance of methane-reducing practices by producers. In this study we explored the changes in plasma profiles associated with the reduction of methane emissions. Plasma samples were collected from lactating primiparous Holstein cows fed the same diet with (Treated, n = 12) or without (Control, n = 13) an anti-methanogenic feed additive for six weeks. Daily methane emissions (CH(4), g/d) were reduced by 23% in the Treated group with no changes in milk production, feed intake, body weight, and biochemical indicators of health status. Plasma metabolome analyses were performed using untargeted [nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS)] and targeted (LC-MS/MS) approaches. We identified 48 discriminant metabolites. Some metabolites mainly of microbial origin such as dimethylsulfone, formic acid and metabolites containing methylated groups like stachydrine, can be related to rumen methanogenesis and can potentially be used as markers. The other discriminant metabolites are produced by the host or have a mixed microbial-host origin. These metabolites, which increased in treated cows, belong to general pathways of amino acids and energy metabolism suggesting a systemic non-negative effect on the animal" |
| Keywords: | Animals Body Weight Cattle Diet/veterinary Energy Metabolism Intestinal Mucosa/*metabolism *Metabolome Methane/*analysis/*biosynthesis Milk Proteins/*metabolism; |
| Notes: | "MedlineYanibada, Benedict Hohenester, Ulli Petera, Melanie Canlet, Cecile Durand, Stephanie Jourdan, Fabien Boccard, Julien Martin, Cecile Eugene, Maguy Morgavi, Diego P Boudra, Hamid eng Research Support, Non-U.S. Gov't England 2020/09/26 Sci Rep. 2020 Sep 24; 10(1):15591. doi: 10.1038/s41598-020-72145-w" |
