| Title: | Identification and Characterization of Terpene Synthases Potentially Involved in the Formation of Volatile Terpenes in Carrot (Daucus carota L.) Roots |
| Author(s): | Yahyaa M; Tholl D; Cormier G; Jensen R; Simon PW; Ibdah M; |
| Address: | "daggerNewe Ya'ar Research Center, Agriculture Research Organization, Post Office Box 1021, Ramat Yishay 30095, Israel. double daggerDepartment of Biological Sciences, Virginia Polytechnic Institute and State University, 409 Latham Hall, 220 Agquad Lane, Blacksburg, Virginia 24061, United States. section signDepartment of Biological Sciences, Virginia Polytechnic Institute and State University, 119 Life Sciences I, 970 Washington Street, Blacksburg, Virginia 24061, United States. parallelVegetable Crops Research Unit, Department of Horticulture, University of Wisconsin-Madison, 1575 Linden Drive, Madison, Wisconsin 53706, United States" |
| ISSN/ISBN: | 1520-5118 (Electronic) 0021-8561 (Linking) |
| Abstract: | "Plants produce an excess of volatile organic compounds, which are important in determining the quality and nutraceutical properties of fruit and root crops, including the taste and aroma of carrots (Daucus carota L.). A combined chemical, biochemical, and molecular study was conducted to evaluate the differential accumulation of volatile terpenes in a diverse collection of fresh carrots (D. carota L.). Here, we report on a transcriptome-based identification and functional characterization of two carrot terpene synthases, the sesquiterpene synthase, DcTPS1, and the monoterpene synthase, DcTPS2. Recombinant DcTPS1 protein produces mainly (E)-beta-caryophyllene, the predominant sesquiterpene in carrot roots, and alpha-humulene, while recombinant DcTPS2 functions as a monoterpene synthase with geraniol as the main product. Both genes are differentially transcribed in different cultivars and during carrot root development. Our results suggest a role for DcTPS genes in carrot aroma biosynthesis" |
| Keywords: | Alkyl and Aryl Transferases/genetics/*metabolism Daucus carota/chemistry/*enzymology/genetics/metabolism Plant Proteins/genetics/*metabolism Plant Roots/chemistry/enzymology/genetics/metabolism Terpenes/analysis/*metabolism Volatile Organic Compounds/anal; |
| Notes: | "MedlineYahyaa, Mosaab Tholl, Dorothea Cormier, Guy Jensen, Roderick Simon, Philipp W Ibdah, Mwafaq eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2015/05/01 J Agric Food Chem. 2015 May 20; 63(19):4870-8. doi: 10.1021/acs.jafc.5b00546. Epub 2015 May 7" |
