| Title: | Antenna-Biased Odorant Receptor HvarOR25 in Hippodamia variegata Tuned to Allelochemicals from Hosts and Habitat Involved in Perceiving Preys |
| Author(s): | Xie J; Liu T; Yi C; Liu X; Tang H; Sun Y; Shi W; Khashaveh A; Zhang Y; |
| Address: | "State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Department of Entomology, China Agricultural University, Beijing 100193, China. College of Plant Protection, Hebei Agricultural University, Baoding 071001, China. Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Diseases, College of Life Sciences, Anhui Normal University, Wuhu 241000, China" |
| ISSN/ISBN: | 1520-5118 (Electronic) 0021-8561 (Linking) |
| Abstract: | "Odorant receptors (ORs) of ladybird Hippodamia variegata play vital chemosensory roles in searching and locating preys. In the current study, 37 ORs were initially identified from the antennal transcriptome of H. variegata. The quantitative polymerase chain reaction demonstrated that several HvarORs including HvarOR25 were specific or enriched in ladybird antennae. In two-electrode voltage clamp recordings, recombinant HvarOR25 was narrowly tuned to six chemical ligands including aphid-induced, aphid-derived, and plant-derived volatiles. In electroantennogram assays, all six volatiles elicited electrophysiological responses. Among the six volatiles, cis-3-hexenyl acetate, hexyl butyrate, hexyl hexanoate, and 3-methyl-3-buten-1-ol were attractive for both sexes of H. variegata. Additionally, molecular docking indicated that HvarOR25 was bound to all ligands with high binding affinities. Taken together, HvarOR25 facilitates perception of preys by recognizing relevant allelochemicals from hosts and habitat. Our findings provide valuable insights into understanding biological functions of HvarORs and help to develop a novel biocontrol strategy based on olfactory-active compounds" |
| Keywords: | "Animals Arthropod Antennae *Coleoptera Ecosystem Female Insect Proteins/genetics Male Molecular Docking Simulation Pheromones *Receptors, Odorant/genetics EAG assay Hippodamia variegata allelochemical chemoreception ligand binding odorant receptor two-ele;" |
| Notes: | "MedlineXie, Jiaoxin Liu, Tinghui Yi, Chaoqun Liu, Xiaoxu Tang, Haoyu Sun, Yang Shi, Wangpeng Khashaveh, Adel Zhang, Yongjun eng 2022/01/25 J Agric Food Chem. 2022 Feb 2; 70(4):1090-1100. doi: 10.1021/acs.jafc.1c05593. Epub 2022 Jan 24" |
