| Title: | Molecular cloning and expression of Chimonanthus praecox farnesyl pyrophosphate synthase gene and its possible involvement in the biosynthesis of floral volatile sesquiterpenoids |
| Address: | "Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, PR China" |
| DOI: | 10.1016/j.plaphy.2010.08.015 |
| ISSN/ISBN: | 1873-2690 (Electronic) 0981-9428 (Linking) |
| Abstract: | "Farnesyl pyrophosphate (FPP) synthase catalyzes the biosynthesis of FPP, which is the precursors of sesquiterpenoids such as floral scent volatiles, from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP). cDNA encoding wintersweet (Chimonanthus praecox L.) FPP synthase was isolated by the RT-PCR and RACE methods. The deduced amino acid sequence showed a high identity to plant FPP synthases. Expression of the gene in Escherichia coli yielded FPPS activity that catalyzed the synthesis of FPP as a main product. Tissue-specific and developmental analyses of the mRNA levels of CpFPPS and volatile sesquiterpenoids levels in C. praecox flowers revealed that the FPPS may play a regulatory role in floral volatile sesquiterpenoids of wintersweet" |
| Keywords: | "Amino Acid Sequence Calycanthaceae/*genetics/metabolism DNA, Complementary/analysis Escherichia coli/genetics/metabolism Flowers/*metabolism *Gene Expression *Genes, Plant Geranyltranstransferase/*genetics/metabolism Molecular Sequence Data Oils, Volatile;" |
| Notes: | "MedlineXiang, Lin Zhao, Kaige Chen, Longqing eng Research Support, Non-U.S. Gov't France 2010/09/22 Plant Physiol Biochem. 2010 Oct-Nov; 48(10-11):845-50. doi: 10.1016/j.plaphy.2010.08.015. Epub 2010 Sep 9" |
