| Title: | Adaptation of Helicoverpa armigera to Soybean Peptidase Inhibitors Is Associated with the Transgenerational Upregulation of Serine Peptidases |
| Author(s): | Velasquez-Vasconez PA; Hunt BJ; Dias RO; Souza TP; Bass C; Silva-Filho MC; |
| Address: | "Departamento de Genetica, Escola Superior de Agricultura Luiz de Queiroz, Universidade de Sao Paulo, Av. Padua Dias, 11, Piracicaba 13418-900, SP, Brazil. College of Life and Environmental Sciences, Biosciences, University of Exeter, Penryn Campus, Penryn, Cornwall TR10 9FE, UK. Instituto de Ciencias Biologicas, Universidade Federal de Goias, Goiania 74690-900, GO, Brazil" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "Molecular phenotypes induced by environmental stimuli can be transmitted to offspring through epigenetic inheritance. Using transcriptome profiling, we show that the adaptation of Helicoverpa armigera larvae to soybean peptidase inhibitors (SPIs) is associated with large-scale gene expression changes including the upregulation of genes encoding serine peptidases in the digestive system. Furthermore, approximately 60% of the gene expression changes induced by SPIs persisted in the next generation of larvae fed on SPI-free diets including genes encoding regulatory, oxidoreductase, and protease functions. To investigate the role of epigenetic mechanisms in regulating SPI adaptation, the methylome of the digestive system of first-generation larvae (fed on a diet with and without SPIs) and of the progeny of larvae exposed to SPIs were characterized. A comparative analysis between RNA-seq and Methyl-seq data did not show a direct relationship between differentially methylated and differentially expressed genes, while trypsin and chymotrypsin genes were unmethylated in all treatments. Rather, DNA methylation potential epialleles were associated with transcriptional and translational controls; these may play a regulatory role in the adaptation of H. armigera to SPIs. Altogether, our findings provided insight into the mechanisms of insect adaptation to plant antiherbivore defense proteins and illustrated how large-scale transcriptional reprograming of insect genes can be transmitted across generations" |
| Keywords: | Animals *Soybeans/genetics/metabolism Protease Inhibitors/pharmacology Up-Regulation Serine Proteases/metabolism *Moths/genetics/metabolism Chymotrypsin/genetics/metabolism Trypsin/metabolism Larva/genetics/metabolism Serine/metabolism epigenetics evoluti; |
| Notes: | "MedlineVelasquez-Vasconez, Pedro A Hunt, Benjamin J Dias, Renata O Souza, Thais P Bass, Chris Silva-Filho, Marcio C eng 2014/50275-9/Sao Paulo Research Foundation/ FINANCE CODE 001/Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/ 2019/15488-5/Sao Paulo Research Foundation/ Switzerland 2022/11/27 Int J Mol Sci. 2022 Nov 18; 23(22):14301. doi: 10.3390/ijms232214301" |
