« Previous AbstractThe impact of O(2)/Ar ratio on morphology and functional properties in reactive sputtering of metal oxide thin films    Next AbstractIdentification of stimuli that mediate experience-dependent modification of homosexual courtship in Drosophila melanogaster »

Appl Environ Microbiol

Title:		Improved secretion of native human insulin-like growth factor 1 from gas1 mutant Saccharomyces cerevisiae cells
Author(s):		Vai M; Brambilla L; Orlandi I; Rota N; Ranzi BM; Alberghina L; Porro D;
Address:		"Dipartimento di Biotecnologie e Bioscienze, Universita degli Studi di Milano-Bicocca, 20126 Milan, Italy"
Journal Title:		Appl Environ Microbiol
Year:		2000
Volume:		66
Issue:		12
Page Number:		5477 - 5479
DOI:		10.1128/AEM.66.12.5477-5479.2000
ISSN/ISBN:		0099-2240 (Print) 1098-5336 (Electronic) 0099-2240 (Linking)
Abstract:		"We studied the secretion of recombinant human insulin-like growth factor 1 (rhIGF-1) from transformed yeast cells. The hIGF-1 gene was fused to the mating factor alpha prepro- leader sequence under the control of the constitutive ACT1 promoter. We found that the inactivation of the GAS1 gene in the host strain led to a supersecretory phenotype yielding a considerable increase, from 8 to 55 mg/liter, in rhIGF-1 production"
Keywords:		"Fungal Proteins/*genetics Genes, Fungal Humans Insulin-Like Growth Factor I/*metabolism Mating Factor Membrane Glycoproteins/*genetics Mutation Peptides/genetics Phenotype Recombinant Proteins/metabolism Saccharomyces cerevisiae/*genetics/*physiology *Sac;"
Notes:		"MedlineVai, M Brambilla, L Orlandi, I Rota, N Ranzi, B M Alberghina, L Porro, D eng 2000/12/01 Appl Environ Microbiol. 2000 Dec; 66(12):5477-9. doi: 10.1128/AEM.66.12.5477-5479.2000"