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Comput Struct Biotechnol J

Title:		Gut transcriptome of two bark beetle species stimulated with the same kairomones reveals molecular differences in detoxification pathways
Author(s):		Torres-Banda V; Obregon-Molina G; Viridiana Soto-Robles L; Albores-Medina A; Fernanda Lopez M; Zuniga G;
Address:		"Laboratorio de Variacion Biologica y Evolucion, Departamento de Zoologia, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Prolongacion de Carpio y Plan de Ayala s/n, Miguel Hidalgo, Mexico City, CP 11340, Mexico. Departamento de Toxicologia, Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional, Av. Instituto Politecnico Nacional 2508, San Pedro Zacatenco, Gustavo A. Madero, Mexico City, CP 07360, Mexico"
Journal Title:		Comput Struct Biotechnol J
Year:		2022
Volume:		20220616
Issue:
Page Number:		3080 - 3095
DOI:		10.1016/j.csbj.2022.06.029
ISSN/ISBN:		2001-0370 (Print) 2001-0370 (Electronic) 2001-0370 (Linking)
Abstract:		"Dendroctonus bark beetles are the most destructive agents in coniferous forests. These beetles come into contact with the toxic compounds of their host's chemical defenses throughout their life cycle, some of which are also used by the insects as kairomones to select their host trees during the colonization process. However, little is known about the molecular mechanisms by which the insects counteract the toxicity of these compounds. Here, two sibling species of bark beetles, D. valens and D. rhizophagus, were stimulated with vapors of a blend of their main kairomones (alpha-pinene, beta-pinene and 3-carene), in order to compare the transcriptional response of their gut. A total of 48 180 unigenes were identified in D. valens and 43 704 in D. rhizophagus, in response to kairomones blend. The analysis of differential gene expression showed a transcriptional response in D. valens (739 unigenes, 0.58-10.36 Log2FC) related to digestive process and in D. rhizophagus (322 unigenes 0.87-13.08 Log2FC) related to xenobiotics metabolism. The expression profiles of detoxification genes mainly evidenced the up-regulation of COEs and GSTs in D. valens, and the up-regulation of P450s in D. rhizophagus. Results suggest that terpenes metabolism comes accompanied by an integral hormetic response, result of compensatory mechanisms, including the activation of other metabolic pathways, to ensure the supply of energy and the survival of organisms which is specific for each species, according to its life history and ecological strategy"
Keywords:		Carboxylesterase Cytochrome P450 monooxygenases Dendroctonus rhizophagus Dendroctonus valens Detoxification process Glutathione S-transferase;
Notes:		"PubMed-not-MEDLINETorres-Banda, Veronica Obregon-Molina, Gabriel Viridiana Soto-Robles, L Albores-Medina, Arnulfo Fernanda Lopez, Maria Zuniga, Gerardo eng Netherlands 2022/07/06 Comput Struct Biotechnol J. 2022 Jun 16; 20:3080-3095. doi: 10.1016/j.csbj.2022.06.029. eCollection 2022"