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Insect Mol Biol

Title:		"An odorant receptor mediates the attractiveness of cis-jasmone to Campoletis chlorideae, the endoparasitoid of Helicoverpa armigera"
Author(s):		Sun YL; Dong JF; Ning C; Ding PP; Huang LQ; Sun JG; Wang CZ;
Address:		"State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. Forestry College, Henan University of Science and Technology, Luoyang, Henan Province, China. Biology and Food Engineering College, Anyang Institute of Technology, Anyang, Henan Province, China. College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China"
Journal Title:		Insect Mol Biol
Year:		2019
Volume:		20181005
Issue:		1
Page Number:		23 - 34
DOI:		10.1111/imb.12523
ISSN/ISBN:		1365-2583 (Electronic) 0962-1075 (Linking)
Abstract:		"Parasitic wasps rely on olfaction to locate their hosts in complex chemical environments. Odorant receptors (ORs) function together with well-conserved odorant coreceptors (ORcos) to determine the sensitivity and specificity of olfactory reception. Campoletis chlorideae (Hymenoptera: Ichneunmonidae) is a solitary larval endoparasitoid of the cotton bollworm, Helicoverpa armigera, and some other noctuid species. To understand the molecular basis of C. chlorideae's olfactory reception, we sequenced the transcriptome of adult male and female heads (including antennae) and identified 211 OR transcripts, with 95 being putatively full length. The tissue expression profiles, as assessed by reverse-transcription PCR, showed that seven ORs were expressed only or more highly in female antennae. Their functions were analysed using the Xenopu slaevis oocyte expression system and two-electrode voltage-clamp recordings. CchlOR62 was tuned to cis-jasmone, which was attractive to female C. chlorideae adults and H. armigera larvae in the subsequent behavioural assays. Further bioassays using caged plants showed that the parasitism rate of H. armigera larvae by C. chlorideae on cis-jasmone-treated tobacco plants was higher than on the control plants. Thus, cis-jasmone appears to be an important infochemical involved in the interactions of plants, H. armigera and C. chlorideae, and CchlOR62 mediates the attractiveness of cis-jasmone to C. chlorideae"
Keywords:		"Animals Arthropod Antennae/metabolism Cyclopentanes/*metabolism Female *Host-Parasite Interactions Larva/metabolism/parasitology Male Moths/metabolism/*parasitology Oxylipins/*metabolism Pest Control, Biological Receptors, Odorant/*metabolism Smell Tobacc;"
Notes:		"MedlineSun, Y-L Dong, J-F Ning, C Ding, P-P Huang, L-Q Sun, J-G Wang, C-Z eng Research Support, Non-U.S. Gov't England 2018/07/31 Insect Mol Biol. 2019 Feb; 28(1):23-34. doi: 10.1111/imb.12523. Epub 2018 Oct 5"