| Title: | The main component of the aphid alarm pheromone (E)-beta-farnesene affects the growth and development of Spodoptera exigua by mediating juvenile hormone-related genes |
| Author(s): | Sun Y; Li Y; Zhang W; Jiang B; Tao SM; Dai HY; Xu XT; Sun YX; Yang L; Zhang YJ; |
| Address: | "Key Laboratory for Conservation and Use of Important Biological Resources of Anhui Province, Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Diseases, College of Life Sciences, Anhui Normal University, Wuhu, China. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, China. Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China" |
| ISSN/ISBN: | 1664-462X (Print) 1664-462X (Electronic) 1664-462X (Linking) |
| Abstract: | "The (E)-beta-farnesene (EbetaF) is one of the most important secondary metabolites in some plants and provides indirect defense against aphids. However, the direct effect of EbetaF against pests is still unclear. In this study, various concentrations of EbetaF (0.16, 0.8, and 4 g/kg) were provided in an artificial diet to determine the direct effects of EbetaF on Spodoptera exigua. The results showed that an artificial diet containing 4 g/kg of EbetaF reduced the final survival of the S. exigua larvae and per female fecundity of adults significantly when compared with CK and SC controls (p < 0.05), then ultimately it also significantly affected the intrinsic rate of increase (p < 0.05). Furthermore, the results of the EbetaF bioassay in an artificial diet also indicated that the proliferation of the S. exigua population was inhibited by the ingestion of EbetaF in a dose-dependent manner. Combined differential RNA-seq data and RT-qPCR analysis, it was found that four key genes involved in juvenile hormone degradation significantly upregulated in S. exigua larvae treated by EbetaF at a dose of 0.8 and 4 g/kg when compared with two controls (p < 0.05). This indicated that EbetaF could disturb the normal function of juvenile hormones and reduce the survival rate of S. exigua larvae. Additionally, two key genes that regulate per fecundity of S. exigua females, including SeVg and SeVgR, were significantly downregulated in adult females (p < 0.05) when they were treated with 0.8 and 4 g/kg of EbetaF at the larval stage, relative to the expression of these genes after treatment with controls. These findings suggested that EbetaF first disturbed the normal function of juvenile hormone by upregulating key degradation genes, and then inhibited the expression of SeVg/SeVgR genes and proteins, thus reducing the population size of S. exigua by increasing larval mortality and inhibiting per female fecundity" |
| Keywords: | (E)-beta-farnesene RNA-seq SeVg and SeVgR Spodoptera exigua reproduction and oviposition; |
| Notes: | "PubMed-not-MEDLINESun, Yang Li, Yan Zhang, Wen Jiang, Bin Tao, Si-Min Dai, Han-Yang Xu, Xin-Tong Sun, Yue-Xin Yang, Lei Zhang, Yong-Jun eng Switzerland 2022/09/10 Front Plant Sci. 2022 Aug 23; 13:863626. doi: 10.3389/fpls.2022.863626. eCollection 2022" |
