| Title: | Comprehensive analysis of floral scent and fatty acids in nectar of Silene nutans through modern analytical gas chromatography techniques |
| Author(s): | Spadafora ND; Eggermont D; Krestakova V; Chenet T; Van Rossum F; Purcaro G; |
| Address: | "Department of Chemical, Pharmaceutical and Agricultural Sciences, University of Ferrara, 44121, Ferrara, Italy. Gembloux Agro-Bio Tech, University of Liege, Passage des Deportes 2, Gembloux, 5030, Belgium. Gembloux Agro-Bio Tech, University of Liege, Passage des Deportes 2, Gembloux, 5030, Belgium; Department of Biochemistry, Faculty of Science, Masaryk University, 32500, Brno, Czech Republic. Department of Environment and Prevention Sciences, University of Ferrara, 44121, Ferrara, Italy. Meise Botanic Garden, Nieuwelaan 38, 1860, Meise, Belgium; Service general de l'Enseignement superieur et de la Recherche scientifique, Federation Wallonie-Bruxelles, rue A. Lavallee 1, 1080, Brussels, Belgium. Gembloux Agro-Bio Tech, University of Liege, Passage des Deportes 2, Gembloux, 5030, Belgium. Electronic address: gpurcaro@uliege.be" |
| DOI: | 10.1016/j.chroma.2023.463977 |
| ISSN/ISBN: | 1873-3778 (Electronic) 0021-9673 (Linking) |
| Abstract: | "The aim of this work was to show the potential of multidimensional gas chromatography combined with mass spectrometry and suitable chemometrics means based on untargeted and profiling data analysis to strengthen the information provided by floral scent and nectar fatty acids of four genetically differentiated lineages (E1, W1, W2, and W3) of the nocturnal moth-pollinated herb Silene nutans. Volatile organic compounds emitted by flowers were trapped for a total of 42 samples by in-vivo sampling dynamic head space for analysing floral scent by untargeted approach, while 37 samples of nectar were collected for analysing fatty acids through profiling analysis. The resulting data from floral scent analysis were aligned and compared using a tile-based methodology followed by data mining to access high-level information. Based on floral scent and nectar fatty acid results, it was possible to distinguish E1 from the W lineages, and W3 from W1 and W2. This work puts the bases for a larger study aiming to clarify the existence of prezygotic barriers involved in speciation among lineages of S. nutans, and thus the possible implication of different flower scents and nectar compositions in this phenomenon" |
| Keywords: | Plant Nectar/analysis Odorants/analysis *Silene Gas Chromatography-Mass Spectrometry Flowers/chemistry *Volatile Organic Compounds/analysis Chemometrics Fatty acids GC x GC Head-space extraction Volatile organic compounds; |
| Notes: | "MedlineSpadafora, Natasha Damiana Eggermont, Damien Krestakova, Veronika Chenet, Tatiana Van Rossum, Fabienne Purcaro, Giorgia eng Netherlands 2023/04/14 J Chromatogr A. 2023 May 10; 1696:463977. doi: 10.1016/j.chroma.2023.463977. Epub 2023 Apr 7" |
