| Title: | Aphid feeding activates expression of a transcriptome of oxylipin-based defense signals in wheat involved in resistance to herbivory |
| Author(s): | Smith CM; Liu X; Wang LJ; Liu X; Chen MS; Starkey S; Bai J; |
| Address: | "Department of Entomology, Kansas State University, Manhattan, KS 66506, USA. cmsmith@ksu.edu" |
| DOI: | 10.1007/s10886-010-9756-8 |
| ISSN/ISBN: | 1573-1561 (Electronic) 0098-0331 (Print) 0098-0331 (Linking) |
| Abstract: | "Damage by the Russian wheat aphid (RWA), Diuraphis noxia, significantly reduces wheat and barley yields worldwide. In compatible interactions, virulent RWA populations flourish and susceptible plants suffer extensive leaf chlorophyll loss. In incompatible interactions, RWA reproduction and population growth are significantly reduced and RWA-related chlorophyll loss in resistant plants is minor. The objectives of this study were to develop an understanding of the molecular and phytochemical bases of RWA resistance in plants containing the Dnx resistance gene. Microarray, real-time polymerase chain reaction, and phytohormone assays were conducted to identify transcriptome components unique to RWA-infested Dnx plants and susceptible (Dn0) plants, and to identify and characterize putative genes involved in Dnx plant defense responses. We found that RWA-infested Dnx plants upregulated >180 genes related to reactive oxygen species, signaling, pathogen defense, and arthropod allelochemical and physical defense. The expression of several of these genes in RWA-infested Dnx plants increased significantly from 6- to 24-h post infestation (hpi), but their expression in Dn0 plants, when present, was delayed until 48- to 96 hpi. Concentrations of 16- and 18-carbon fatty acids, trans-methyl-12-oxophytodienoic acid, and abscisic acid were significantly greater in Dnx foliage than in Dn0 foliage after RWA infestation, suggesting that Dnx RWA defense and resistance genes may be regulated via the oxylipin pathway. These findings provide a foundation for the elucidation of the molecular basis for compatible- and incompatible plant-aphid interactions" |
| Keywords: | "Animal Feed Animals Aphids/*physiology *Gene Expression Profiling Gene Expression Regulation, Plant Genotype Oxylipins/*metabolism Phenotype Pheromones/metabolism Reactive Oxygen Species/metabolism Signal Transduction Time Factors Triticum/*genetics/metab;" |
| Notes: | "MedlineSmith, Charles Michael Liu, Xuming Wang, Liang J Liu, Xiang Chen, Ming-Shun Starkey, Sharon Bai, Jianfa eng P20 GM103418/GM/NIGMS NIH HHS/ P20 RR016475/RR/NCRR NIH HHS/ P20RR16475/RR/NCRR NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2010/03/17 J Chem Ecol. 2010 Mar; 36(3):260-76. doi: 10.1007/s10886-010-9756-8. Epub 2010 Feb 21" |
