| Title: | Delineation of functional regions within the subunits of the Saccharomyces cerevisiae cell adhesion molecule a-agglutinin |
| Author(s): | Shen ZM; Wang L; Pike J; Jue CK; Zhao H; de Nobel H; Kurjan J; Lipke PN; |
| Address: | "Department of Biological Sciences and the Institute for Biomolecular Structure and Function, Hunter College of the City University of New York, New York 10021, USA" |
| ISSN/ISBN: | 0021-9258 (Print) 0021-9258 (Linking) |
| Abstract: | "a-Agglutinin from Saccharomyces cerevisiae is a cell adhesion glycoprotein expressed on the surface of cells of a mating type and consists of an anchorage subunit Aga1p and a receptor binding subunit Aga2p. Cell wall attachment of Aga2p is mediated through two disulfide bonds to Aga1p (Cappellaro, C., Baldermann, C., Rachel, R., and Tanner, W. (1994) EMBO J. 13, 4737-4744). We report here that purified Aga2p was unstable and had low molar specific activity relative to its receptor alpha-agglutinin. Aga2p co-expressed with a 149-residue fragment of Aga1p formed a disulfide-linked complex with specific activity 43-fold higher than Aga2p expressed alone. Circular dichroism of the complex revealed a mixed alpha/beta structure, whereas Aga2p alone had no periodic secondary structure. A 30-residue Cys-rich Aga1p fragment was partially active in stabilization of Aga2p activity. Mutation of either or both Aga2p cysteine residues eliminated stabilization of Aga2p. Thus the roles of Aga1p include both cell wall anchorage and cysteine-dependent conformational restriction of the binding subunit Aga2p. Mutagenesis of AGA2 identified only C-terminal residues of Aga2p as being essential for binding activity. Aga2p residues 45-72 are similar to sequences in soybean Nod genes, and include residues implicated in interactions with both Aga1p (including Cys(68)) and alpha-agglutinin" |
| Keywords: | "Agglutinins/chemistry/physiology Amino Acid Sequence Binding Sites Cell Wall/physiology Cloning, Molecular Disulfides Mating Factor Molecular Sequence Data Mutagenesis, Site-Directed Peptides/*chemistry/genetics/*physiology Protein Conformation Protein Su;" |
| Notes: | "MedlineShen, Z M Wang, L Pike, J Jue, C K Zhao, H de Nobel, H Kurjan, J Lipke, P N eng 1RO1-GM47176/GM/NIGMS NIH HHS/ 2S060654/PHS HHS/ RR-03037/RR/NCRR NIH HHS/ Research Support, U.S. Gov't, P.H.S. 2001/03/30 J Biol Chem. 2001 May 11; 276(19):15768-75. doi: 10.1074/jbc.M010421200. Epub 2001 Feb 5" |
