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Curr Genet

Title:		Cloning and characterization of a Schizophyllum gene with A beta 6 mating-type activity
Author(s):		Shen GP; Park DC; Ullrich RC; Novotny CP;
Address:		"Department of Microbiology and Molecular Genetics, University of Vermont, Burlington 05405, USA"
Journal Title:		Curr Genet
Year:		1996
Volume:		29
Issue:		2
Page Number:		136 - 142
DOI:		10.1007/BF02221577
ISSN/ISBN:		0172-8083 (Print) 0172-8083 (Linking)
Abstract:		"The A-pathway of development in the basidiomycete fungus Schizophyllum commune may be activated by either of two mating-type loci, A alpha and A beta. A alpha consists of two multiallelic genes, Y and Z. Y contains a putative homeodomain; Z contains a homeodomain-related region. Non-self combinations of Y and Z form heteromultimers which are thought to be transcription factors of developmental genes. To more completely understand A-regulated development it is necessary to address the issue of functional redundancy, i.e., how do two different mating loci, A alpha and A beta, both manage to regulate the same pathway. Here we report the structure of a gene with A beta 6 activity. This gene, denoted A beta V6, encodes a deduced polypeptide of 640 amino-acids with a homeodomain motif. V6 also contains a 20-amino acid sequence that is conserved in A alpha Y1, Y3 and Y4. Except for the homeodomain and the conserved sequence, the deduced V6 polypeptide shows no significant identity to A alpha Y, A alpha Z, or other known proteins. The presence of a homeodomain suggests that V, like Y and Z, may be a regulatory protein for genes in the A-pathway. Thus while A alpha and A beta encode different proteins, the general mechanism by which A alpha and A beta components signal A-regulated development may be similar"
Keywords:		"Amino Acid Sequence Base Sequence Blotting, Northern Cloning, Molecular Conserved Sequence Fungal Proteins/*chemistry/*genetics *Genes, Fungal *Genes, Mating Type, Fungal Homeodomain Proteins/*chemistry/*genetics Lipoproteins/genetics Molecular Sequence D;"
Notes:		"MedlineShen, G P Park, D C Ullrich, R C Novotny, C P eng GM34023/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1996/01/01 Curr Genet. 1996 Jan; 29(2):136-42. doi: 10.1007/BF02221577"