| Title: | Laser desorption mass spectrometry of synthetic lipid A-like compounds |
| Author(s): | Seydel U; Lindner B; Zahringer U; Rietschel ET; Kusumoto S; Shiba T; |
| ISSN/ISBN: | 0306-042X (Print) 0306-042X (Linking) |
| Abstract: | "The applicability and the present limitations of the laser microprobe mass analyser LAMMA -500 as an instrument for the structural analysis of higher molecular weight, non-volatile, bio-organic compounds (less than or equal to 2000 u) were investigated. For this purpose mass spectra of various synthetic and natural compounds representing cell wall components of Gram-negative bacteria, e.g. phospholipids and lipid A-like molecules were studied. In several cases these spectra exhibited relatively simple and interpretable patterns with a prominent quasi-molecular ion originating from alkali attachment. For one group of the compounds studied--synthetic lipid A-like molecules containing a phosphate moiety--the spectra were rather complicated and lacked pronounced quasi-molecular peaks. Possible reasons for this observation are discussed" |
| Keywords: | Bacteria/analysis *Lasers Lipid A/*analysis Mass Spectrometry/*methods Phospholipids/analysis; |
| Notes: | "MedlineSeydel, U Lindner, B Zahringer, U Rietschel, E T Kusumoto, S Shiba, T eng England 1984/03/01 Biomed Mass Spectrom. 1984 Mar; 11(3):132-41. doi: 10.1002/bms.1200110308" |
