| Title: | Electrophysiological Recording from Drosophila Trichoid Sensilla in Response to Odorants of Low Volatility |
| Author(s): | Ng R; Lin HH; Wang JW; Su CY; |
| Address: | "Division of Biological Sciences, University of California San Diego. Division of Biological Sciences, University of California San Diego; c8su@ucsd.edu" |
| ISSN/ISBN: | 1940-087X (Electronic) 1940-087X (Linking) |
| Abstract: | "Insects rely on their sense of smell to guide a wide range of behaviors that are critical for their survival, such as food-seeking, predator avoidance, oviposition, and mating. Myriad chemicals of varying volatilities have been identified as natural odorants that activate insect Olfactory Receptor Neurons (ORNs). However, studying the olfactory responses to low-volatility odorants has been hampered by an inability to effectively present such stimuli using conventional odor-delivery methods. Here, we describe a procedure that permits the effective presentation of low-volatility odorants for in vivo Single-Sensillum Recording (SSR). By minimizing the distance between the odor source and the target tissue, this method allows for the application of biologically salient but hitherto inaccessible odorants, including palmitoleic acid, a stimulatory pheromone with a demonstrated effect on ORNs involved in courtship and mating behavior(1). Our procedure thus affords a new avenue to assay a host of low-volatility odorants for the study of insect olfaction and pheromone communication" |
| Keywords: | Animals Berberine/pharmacology Choline/pharmacology Drosophila/*physiology Electrodes Electrophysiological Phenomena/drug effects Female Odorants Sensilla/drug effects/*physiology Sucrose/pharmacology Video Recording; |
| Notes: | "MedlineNg, Renny Lin, Hui-Hao Wang, Jing W Su, Chih-Ying eng R01 DC009597/DC/NIDCD NIH HHS/ R01 DC015519/DC/NIDCD NIH HHS/ R01 DC016466/DC/NIDCD NIH HHS/ R01 DK092640/DK/NIDDK NIH HHS/ 2017/08/09 J Vis Exp. 2017 Jul 27; (125):56147. doi: 10.3791/56147" |
