| Title: | Acerola (Malpighia glabra L.) and guava (Psidium guayaba L.) industrial processing by-products stimulate probiotic Lactobacillus and Bifidobacterium growth and induce beneficial changes in colonic microbiota |
| Author(s): | Menezes F; de Melo FHC; Vieira ARS; Almeida ETC; Lima MS; Aquino JS; Gomez-Zavaglia A; Magnani M; de Souza EL; |
| Address: | "Laboratory of Food Microbiology, Department of Nutrition, Health Sciences Center, Federal University of Paraiba, Joao Pessoa, Brazil. Laboratory of Microbial Processes in Foods, Department of Food Engineering, Federal University of Paraiba, Joao Pessoa, Brazil. Department of Food Technology, Federal Institute of Sertao de Pernambuco, Petrolina, Brazil. Laboratory of Experimental Nutrition, Department of Nutrition, Health Sciences Center, Federal University of Paraiba, Joao Pessoa, Brazil. Center for Research and Development in Food Cryotechnology (CCT - CONICET La Plata), Buenos Aires, Argentina" |
| ISSN/ISBN: | 1365-2672 (Electronic) 1364-5072 (Linking) |
| Abstract: | "AIMS: This study evaluated whether by-products from industrial processing of acerola (Malpighia glabra L.; AB) and guava (Psidium guajava L.; GB) fruit may stimulate the growth and metabolism of probiotic Lactobacillus and Bifidobacterium and induce changes in human colonic microbiota. METHODS AND RESULTS: The ability of non-digested and digested AB or GB to stimulate the growth ad metabolism of Lactobacillus acidophilus LA-05, Lactobacillus casei L-26 and Bifidobacterium animalis subsp. lactis BB-12 was evaluated. Changes in populations of distinct bacterial groups of human colonic microbiota induced by digested AB and GB were evaluated using an in vitro colonic fermentation system. Non-digested and digested AB and GB favoured probiotic growth. No difference among counts of probiotics in media with glucose, fructooligosaccharides and non-digested and digested AB and GB was found during a 48-h cultivation. Cultivation of probiotics in media with non-digested and digested AB and GB resulted in decreased pH, increased organic acid production and sugar consumption over time. Digested AB and GB caused overall beneficial changes in abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations, besides to decrease the pH and increase the short-chain fatty acid production during a 24-h in vitro colonic fermentation. CONCLUSION: AB and GB could be novel prebiotic ingredients because they can stimulate the growth and metabolism of probiotics and induce overall beneficial changes in human colonic microbiota. SIGNIFICANCE AND IMPACT OF THE STUDY: AB and GB stimulated the growth and metabolism of probiotics, in addition to induce beneficial alterations in human colonic microbiota composition and increase short-chain fatty acid production. These results characterize AB and GB as potential prebiotic ingredients and fruit processing by-products as sources of added-value compounds" |
| Keywords: | "Bifidobacterium animalis/*growth & development/metabolism Clostridiales Colon/*microbiology Fatty Acids, Volatile/metabolism Fermentation Fruit/chemistry/metabolism Gastrointestinal Microbiome Humans Lactobacillus/*growth & development/metabolism Lactobac;" |
| Notes: | "MedlineMenezes, F N D D de Melo, F H C Vieira, A R S Almeida, E T C Lima, M S Aquino, J S Gomez-Zavaglia, A Magnani, M de Souza, E L eng 001/Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/ England 2020/08/19 J Appl Microbiol. 2021 Apr; 130(4):1323-1336. doi: 10.1111/jam.14824. Epub 2020 Sep 23" |
