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Insect Biochem Mol Biol

Title:		"Characterization of acyl-CoA-binding protein (ACBP) in the pheromone gland of the silkworm, Bombyx mori"
Author(s):		Matsumoto S; Yoshiga T; Yokoyama N; Iwanaga M; Koshiba S; Kigawa T; Hirota H; Yokoyama S; Okano K; Mita K; Shimada T; Tatsuki S;
Address:		"The Institute of Physical and Chemical Research (RIKEN), Hirosawa 2-1, Wako-shi, Saitama 351-0198, Japan. smatsu@postman.riken.go.jp"
Journal Title:		Insect Biochem Mol Biol
Year:		2001
Volume:		31
Issue:		6-Jul
Page Number:		603 - 609
DOI:		10.1016/s0965-1748(00)00165-x
ISSN/ISBN:		0965-1748 (Print) 0965-1748 (Linking)
Abstract:		"Various fatty acyl-CoAs are involved as intermediates or precursors of sex pheromone components in the biosynthetic pathway of the pheromones in many lepidopteran insects. We have purified a 10-kDa protein from the cytosolic fraction of Bombyx mori pheromone glands by using affinity chromatography with a palmitoyl-CoA-agarose column and reversed-phase HPLC. Amino acid sequence analysis of the fragment peptides obtained from the purified protein, and a homology search, revealed that this protein was a member of acyl-CoA-binding proteins (ACBPs). MALDI-TOF mass spectral analysis of the purified protein and cloning of the gene from a pheromone gland cDNA library confirmed B. mori ACBP to be a 90 amino acid protein with 78.9% identity to that of Manduca sexta ACBP. The secondary structure of the recombinant B. mori ACBP was determined by NMR spectroscopy. Northern blot analysis demonstrated that B. mori ACBP was predominantly expressed in the pheromone gland and the corresponding transcript was expressed from the day before adult eclosion. Present results suggest that ACBP plays a significant role in the production of sex pheromones regulated by the neurohormone, pheromone biosynthesis activating neuropeptide (PBAN)"
Keywords:		"*Acyl Coenzyme A Amino Acid Sequence Animals Base Sequence Blotting, Northern/methods Bombyx/*chemistry Carrier Proteins/*analysis/genetics Cattle DNA, Complementary Diazepam Binding Inhibitor Humans Molecular Sequence Data Protein Structure, Secondary Se;"
Notes:		"MedlineMatsumoto, S Yoshiga, T Yokoyama, N Iwanaga, M Koshiba, S Kigawa, T Hirota, H Yokoyama, S Okano, K Mita, K Shimada, T Tatsuki, S eng Research Support, Non-U.S. Gov't England 2001/03/27 Insect Biochem Mol Biol. 2001 Apr 27; 31(6-7):603-9. doi: 10.1016/s0965-1748(00)00165-x"