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Insect Biochem Mol Biol

Title:		"Functional characterization of a desaturase from the tobacco hornworm moth (Manduca sexta) with bifunctional Z11- and 10,12-desaturase activity"
Author(s):		Matouskova P; Pichova I; Svatos A;
Address:		"Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo n, 2, 166 10 Prague 6, Czech Republic"
Journal Title:		Insect Biochem Mol Biol
Year:		2007
Volume:		20070312
Issue:		6
Page Number:		601 - 610
DOI:		10.1016/j.ibmb.2007.03.004
ISSN/ISBN:		0965-1748 (Print) 0965-1748 (Linking)
Abstract:		"The pheromone blend produced by the tobacco hornworm moth (Manduca sexta) (L.) female is unusually complex and contains two conjugated dienals and trienals together with two monounsaturated alkenals. Here, we describe the identification and construction of two genes encoding MsexKPSE and MsexAPTQ desaturases from a cDNA library prepared from the total RNA of the M. sexta pheromone gland. The MsexKPSE desaturase shares a high degree of similarity with Delta(9)-desaturases from different moth species. The functional expression of MsexAPTQ desaturase in Saccharomyces cerevisiae followed by a detailed GC-MS analysis of fatty acid methyl esters (FAME) and their derivatized products and gas-phase Fourier transform infrared (FTIR) spectroscopy of the extracted FAME confirms that this enzyme is a bifunctional Z-Delta(11)-desaturase. MsexAPTQ desaturase catalyses the production of Z11-hexadecenoate (Z11-16) and Z10E12- and E10E12-hexadecadienoates (Z10E12-16) via 1,4-desaturation of the Z11-16 substrate. The stereochemistry of 1,4-desaturation and formation of isomers is discussed"
Keywords:		"Amino Acid Sequence Animals Cloning, Molecular DNA, Complementary Fatty Acid Desaturases/chemistry/*genetics/*metabolism Female Gas Chromatography-Mass Spectrometry Gene Library Manduca/*enzymology/metabolism Phylogeny Sequence Alignment Sequence Analysis;"
Notes:		"MedlineMatouskova, Petra Pichova, Iva Svatos, Ales eng Research Support, Non-U.S. Gov't England 2007/05/23 Insect Biochem Mol Biol. 2007 Jun; 37(6):601-10. doi: 10.1016/j.ibmb.2007.03.004. Epub 2007 Mar 12"