| Title: | A novel method to detect bovine sex pheromones using l-tyrosine-capped silver nanoparticles: Special reference to nanosensor based estrus detection |
| Author(s): | Manikkaraja C; Mahboob S; Al-Ghanim KA; Rajesh D; Selvaraj K; Sivakumar M; Al-Misned F; Ahmed Z; Archunan G; |
| Address: | "Pheromone Technology Lab, Department of Animal Science, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India. Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. Pheromone Technology Lab, Department of Animal Science, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India; Research Institute in Semiochemistry and Applied Ethology (IRSEA), Quartier Salignan, 84400 APT, France. Division of Nanoscience and Technology, Anna University-BIT Campus, Tiruchirappalli 620024, Tamil Nadu, India. Pheromone Technology Lab, Department of Animal Science, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India. Electronic address: archunan@bdu.ac.in" |
| DOI: | 10.1016/j.jphotobiol.2019.111747 |
| ISSN/ISBN: | 1873-2682 (Electronic) 1011-1344 (Linking) |
| Abstract: | "In the present study, a simple and a selective colorimetric method for pheromone detection to diagnose estrus in cattle was established based on the l-tyrosine functionalized silver nanoparticles (l-TyrAgNPs). The synthesized silver nanoparticles was spotted by color change (colorless to pale yellow) due to surface plasmon resonance (SPR). In order to confirm, Ag nanoparticles was characterized by field emission scanning electron microscopy (FESEM), dynamic light scattering (DLS) and zeta potential, X-ray diffraction (XRD) and UV- Vis spectrophotometer. It was found that the pre-colored Ag colloids could be turned from yellow to reddish-brown by the addition of the sex pheromones such as acetic acid or propionic acid, which may have potential application in the colorimetric sensor. The augmented optical nature of nanoparticles furnishes a suitable base to develop a colorimetric sensor for bovine sex pheromones detection. In addition, the computational analyses are critically required to validate residual interactions of bovine odorant-binding protein (OBP) with pheromones. The method was successfully applied to the detection of acetic acid or propionic acid using a biological molecule l-Tyr AgNPs. These results clearly indicate that the biosynthesis of l-Tyr AgNPs can be used as a promising colorimetric sensor for accurate time of estrus prediction in bovine" |
| Keywords: | "Animals Binding Sites Biosensing Techniques/*methods Cattle Colorimetry Estrus Detection/methods Metal Nanoparticles/*chemistry Receptors, Odorant/chemistry/metabolism Sex Attractants/*analysis Silver/*chemistry Tyrosine/*chemistry Ag nanoparticle Bovine;" |
| Notes: | "MedlineManikkaraja, Chidhambaram Mahboob, Shahid Al-Ghanim, Khalid A Rajesh, Durairaj Selvaraj, Kumaresan Sivakumar, Muthusamy Al-Misned, Fahad Ahmed, Zubair Archunan, Govindaraju eng Switzerland 2019/12/31 J Photochem Photobiol B. 2020 Jan; 203:111747. doi: 10.1016/j.jphotobiol.2019.111747. Epub 2019 Dec 17" |
