| Title: | Identification of dynamic microRNA associated with systemic defence against Helicoverpa armigera infestation in Cajanus scarabaeoides |
| Author(s): | Malhotra EV; Jain R; Tyagi S; Venkat Raman K; Bansal S; Pattanayak D; |
| Address: | "ICAR - National Bureau of Plant Genetic Resources, New Delhi, India. ICAR - National Institute for Plant Biotechnology, New Delhi, India" |
| ISSN/ISBN: | 1526-4998 (Electronic) 1526-498X (Linking) |
| Abstract: | "BACKGROUND: Helicoverpa armigera is a major insect pest of several crop plants, including pigeonpea. Resistant gene sources are not available in the cultivated gene pool, but resistance has been observed in its crop wild relative, Cajanus scarabaeoides. Gene regulatory mechanisms governing the systemic immune response of this plant to pod borer infestation have not yet been deciphered. MicroRNA (miRNA) profiles of H. armigera-infested and undamaged adjacent leaves of C. scarabaeoides were compared to gain an insight into the plant-insect interactions and to identify dynamic miRNA molecules potentially acting as mediators of systemic defence responses. RESULTS: A total of 211 conserved, temporally dynamic miRNA were identified in the unfed adjacent leaves, out of which 98 were found to be differentially expressed in comparison to control leaves. On further analysis, most of the miRNA detected in the adjacent leaves was found to target genes involved in the defence pathways and plant immune response. An overlap of the differentially expressing miRNAs was observed between insect-fed and adjacent unfed leaves, indicating the transmission of signal from the site of infestation to the undamaged parts of the plant, indicative of induction of a systemic defence response. CONCLUSION: The miRNA response in the unfed leaves had the signatures of induced changes in metabolism and signal transduction for induction of defence pathway genes. This study reveals the participation of miRNAs in imparting pod borer resistance and mounting a systemic defence response against pod borer infestation in C. scarabaeoides. (c) 2022 Society of Chemical Industry" |
| Keywords: | Animals *Cajanus/chemistry/genetics Larva *MicroRNAs/genetics *Moths/genetics Plant Leaves/genetics Plants/genetics CWRs Cajanus scarabaeoides Helicoverpa armigera herbivory miRNA systemic response; |
| Notes: | "MedlineMalhotra, Era Vaidya Jain, Rishu Tyagi, Saurabh Venkat Raman, K Bansal, Sangita Pattanayak, Debasis eng ECR/2017/000991/Science and Engineering Research Board/ England 2022/04/23 Pest Manag Sci. 2022 Jul; 78(7):3144-3154. doi: 10.1002/ps.6941. Epub 2022 May 24" |
