| Title: | Electrophysiological and behavioral responses of Diaphania glauculalis males to female sex pheromone |
| Author(s): | Ma T; Liu ZT; Zhang YY; Sun ZH; Li YZ; Wen XJ; Chen XY; |
| Address: | "Research Centre for Health Diagnosis and Protection Techniques of Woody Plants, College of Forestry and Landscape Architecture, South China Agricultural University, No. 483, Tianhe District, Guangzhou, China. Research Centre for Health Diagnosis and Protection Techniques of Woody Plants, College of Forestry and Landscape Architecture, South China Agricultural University, No. 483, Tianhe District, Guangzhou, China. wenxiujun@scau.edu.cn" |
| Journal Title: | Environ Sci Pollut Res Int |
| DOI: | 10.1007/s11356-015-4711-4 |
| ISSN/ISBN: | 1614-7499 (Electronic) 0944-1344 (Linking) |
| Abstract: | "The aim of this study is to identify the pheromone active component of female moths, Diaphania glauculalis, an important pest of Anthocephalus chinensis in China. The sex pheromone was extracted from sex pheromone gland extracts of virgin female moth of D. glauculalis using n-hexane, and the pheromone gland extracts of females were analyzed using coupled gas chromatography-electroantennogram detection (GC-EAD) and gas chromatography-mass spectrometry (GC-MS). The sex pheromone active components were based on the comparison the retention time and mass spectrum, with suitable synthetic compounds. (E)-11-hexadecenal (E11-16:Ald) and (E,E)-10,12-hexadecadienal (E10E12-16:Ald) were identified as the major sex pheromone components in the females. Their biological activities were evaluated in a series of electroantennogram (EAG) experiments and four-arm olfactometer assays using synthetic compounds. D. glauculalis males could be attracted by any single component, but a mixture of the E11-16:Ald and E10E12-16:Ald in a ratio of 5:5 elicited a substantial response, demonstrating that the binary blend is essential in male attraction. We therefore conclude that the aldehyde compounds, a mixture of E11-16:Ald and E10E12-16:Ald, comprise the sex pheromone components of D. glauculalis, which might be applied for insect field trapping" |
| Keywords: | "Aldehydes/isolation & purification/pharmacology Alkadienes/isolation & purification/pharmacology Animals Biological Assay China Chromatography, Gas Electrophysiological Phenomena Female Gas Chromatography-Mass Spectrometry Male Mass Spectrometry Moths/*ph;" |
| Notes: | "MedlineMa, Tao Liu, Zhi Tao Zhang, Yuan Yuan Sun, Zhao Hui Li, Yi Zhen Wen, Xiu Jun Chen, Xiao Yang eng Research Support, Non-U.S. Gov't Germany 2015/05/24 Environ Sci Pollut Res Int. 2015 Oct; 22(19):15046-54. doi: 10.1007/s11356-015-4711-4. Epub 2015 May 24" |
