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Mem Inst Oswaldo Cruz

Title:		"More about the role of 2,6-dichlorophenol in tick courtship: identification and olfactometer bioassay in Amblyomma cajennense and Rhipicephalus sanguineus"
Author(s):		Louly CC; Silveira Dda N; Soares SF; Ferri PH; Melo AC; Borges LM;
Address:		"Escola de Veterinaria, Universidade Federal de Goias"
Journal Title:		Mem Inst Oswaldo Cruz
Year:		2008
Volume:		103
Issue:		1
Page Number:		60 - 65
DOI:		10.1590/s0074-02762008000100009
ISSN/ISBN:		0074-0276 (Print) 0074-0276 (Linking)
Abstract:		"This study aimed to identify 2,6-dichlorophenol (2,6-DCP) in Amblyomma cajennense and to evaluate its role in A. cajennense and Rhipicephalus sanguineus courtship. Hexanic extract from attractive females was purified by solid phase extraction and the phenol was identified by the single ion monitoring method using GC/MS. In an olfactometer, the responses of A. cajennense and R. sanguineus males to females, control rubber septa or rubber septa impregnated with 2,6-DCP at 50, 500, and 5000 ng, respectively, were studied. 2,6-DCP was identified in A. cajennense extract and the males oriented themselves toward the concentration of 500 ng. These septa and the females were recognized as copula partners. The septa treated with 2,6-DCP did not attract and were not even recognized by the R. sanguineus males, whereas the females were recognized. Due to the presence of 2,6-DCP in A. cajennense and the results of biological bioassays, it was concluded that this compound acts as an attractant and mounting sex pheromone in this tick, but it does not play any role in R. sanguineus courtship"
Keywords:		"Animals Biological Assay Chlorophenols/isolation & purification/*pharmacology *Courtship Dose-Response Relationship, Drug Female Ixodidae/*drug effects/physiology Male Rhipicephalus sanguineus/drug effects/physiology Sex Attractants/isolation & purificati;"
Notes:		"MedlineLouly, Carla Cristina Braz Silveira, Diana da Nobrega Soares, Sara Fernandes Ferri, Pedro Henrique Melo, Ariane Carla Campos de Borges, Ligia Miranda Ferreira eng Research Support, Non-U.S. Gov't Brazil 2008/03/28 Mem Inst Oswaldo Cruz. 2008 Feb; 103(1):60-5. doi: 10.1590/s0074-02762008000100009"