« Previous AbstractMorphogenic effects of alpha-factor on Saccharomyces cerevisiae a cells    Next Abstract"AG alpha 1 is the structural gene for the Saccharomyces cerevisiae alpha-agglutinin, a cell surface glycoprotein involved in cell-cell interactions during mating" »

J Bacteriol

Title:		Altered immunochemical reactivity of Saccharomyces cerevisiae a-cells after alpha-factor-induced morphogenesis
Author(s):		Lipke PN; Ballou CE;
Address:
Journal Title:		J Bacteriol
Year:		1980
Volume:		141
Issue:		3
Page Number:		1170 - 1177
DOI:		10.1128/jb.141.3.1170-1177.1980
ISSN/ISBN:		0021-9193 (Print) 1098-5530 (Electronic) 0021-9193 (Linking)
Abstract:		"Antibodies were raised against Saccharomyces cerevisiae a-cells that had been exposed to the sex pheromone, alpha-factor. After adsorption of the antiserum with diploid cells, antibodies remained that reacted specifically with the mannan from haploid cells. The characteristic determinant was observed in mannan from pheromone-treated a-cells, in mannan from untreated alpha-cells, and at a much lower concentration, in mannan from control a-cells. The antigens from these three mannans appeared to be identical. The determinant was destroyed by mild-acid hydrolysis or periodate oxidation, but not by proteolysis or digestion with exo-alpha-mannanase. Mutants with altered mannan were unable to express the antigen. Complete acid hydrolysates mannan from alpha-factor-treated a-cells contained mannose, glucose, and N-acetylglucosamine. Partial acid hydrolysis, under conditions that destroyed the antigenic determinant, released only mannose and mannobiose. The mannose fraction was labeled to high specific activity during response of a-cells to alpha-factor if radioactive glucose was the carbon source. Neither alpha- not beta-D-mannopyranosyl phosphate was a hapten. The results are consistent with the presence of a haploid-specific antigen containing an acid-labile mannose determinant and show that the amount of this antigen in a-cell mannan is increased in response to alpha-factor"
Keywords:		"Antigens, Fungal/*analysis Fungal Proteins/*pharmacology Haptens/analysis Hydrolysis Mannans/analysis/*immunology Mating Factor Morphogenesis Mutation *Peptides Polysaccharides/*immunology Saccharomyces cerevisiae/drug effects/*immunology;"
Notes:		"MedlineLipke, P N Ballou, C E eng Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. 1980/03/01 J Bacteriol. 1980 Mar; 141(3):1170-7. doi: 10.1128/jb.141.3.1170-1177.1980"