| Title: | Molecular and genetic evidence for a tetrapolar mating system in Sparassis latifolia |
| Author(s): | Li Y; Yang Y; Huang X; Huang J; Dong C; |
| Address: | "State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100039, China. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. Fujian Rongyi Medicinal Fungus Technology R & D Co., Ltd., Fuzhou, Fujian 350100, China. State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: dongch@im.ac.cn" |
| DOI: | 10.1016/j.funbio.2020.09.005 |
| Abstract: | "Sparassis latifolia is a valuable edible fungus cultivated in East Asia that is rich in beta-glucans. Understanding the mating system and sexual life cycle is important not only for breeding programs to improve strains but also for studies on speciation and population structures. In the present study, mating experiments using monokaryons derived from two different parental strains were performed. Chi-squared test indicated satisfied Mendel segregation, which supported a tetrapolar mating system. A search in the genome for homologs to the well-defined homeodomain and pheromone/receptors, as well as frequently found flanking genes, resulted in the identification of known mating-type loci previously identified in tetrapolar basidiomycetes, each represented by two idiomorphic alleles on separate contigs. Deficiency of the beta-flanking protein in S. latifolia and S. crispa around the MAT-A locus may be explained by the locus being rich in transposable elements adjacent to HD genes. Monokaryotic mycelia are characterized by a slower growth rate and a relative lack of aerial mycelia compared with the parental strain. Chlamydospores can be produced in both monokaryotic and dikaryotic mycelial stages. We provide genetic and molecular evidence for the mating system of S. latifolia, a finding that will be helpful for the cross-breeding of this mushroom" |
| Keywords: | "*Basidiomycota/genetics *Genes, Mating Type, Fungal *Polyporales/genetics Receptors, Pheromone/genetics Cauliflower mushroom Chlamydospore Life cycle Mating system Transposable elements;" |
| Notes: | "MedlineLi, Yongqi Yang, Ying Huang, Xianhua Huang, Jie Dong, Caihong eng Research Support, Non-U.S. Gov't Netherlands 2020/11/21 Fungal Biol. 2020 Dec; 124(12):1004-1012. doi: 10.1016/j.funbio.2020.09.005. Epub 2020 Sep 22" |
