| Title: | Expression plasticity and evolutionary changes extensively shape the sugar-mimic alkaloid adaptation of nondigestive glucosidase in lepidopteran mulberry-specialist insects |
| Author(s): | Li X; Shi L; Dai X; Chen Y; Xie H; Feng M; Chen Y; Wang H; |
| Address: | "College of Animal Sciences, Zhejiang University, Hangzhou, China" |
| ISSN/ISBN: | 1365-294X (Electronic) 0962-1083 (Linking) |
| Abstract: | "During the co-evolutionary arms race between plants and herbivores, insects evolved systematic adaptive plasticity to minimize the chemical defence effects of their host plants. Previous studies mainly focused on the expressional plasticity of enzymes in detoxification and digestion. However, the expressional response and adaptive evolution of other fundamental regulators against host phytochemicals are largely unknown. Glucosidase II (GII), which is composed of a catalytic GIIalpha subunit and a regulatory GIIbeta subunit, is an evolutionarily conserved enzyme that regulates glycoprotein folding. In this study, we found that GIIalpha expression of the mulberry-specialist insect was significantly induced by mulberry leaf extract, 1-deoxynojirimycin (1-DNJ), whereas GIIbeta transcripts were not significantly changed. Moreover, positive selection was detected in GIIalpha when the mulberry-specialist insects diverged from the lepidopteran order, whereas GIIbeta was mainly subjected to purifying selection, thus indicating an asymmetrically selective pressure of GII subunits. In addition, positively selected sites were enriched in the GIIalpha of mulberry-specialist insects and located around the 1-DNJ-binding sites and in the C-terminal region, which could result in conformational changes that affect catalytic activity and substrate-binding efficiency. These results show that expression plasticity and evolutionary changes extensively shape sugar-mimic alkaloids adaptation of nondigestive glucosidase in lepidopteran mulberry-specialist insects. Our study provides novel insights into a deep understanding of the sequestration and adaptation of phytophagous specialists to host defensive compounds" |
| Keywords: | "1-Deoxynojirimycin/metabolism Alkaloids/metabolism Animals Catalytic Domain/genetics *Evolution, Molecular Gene Expression Regulation, Enzymologic/genetics Host-Pathogen Interactions/genetics Lepidoptera/enzymology/*genetics/pathogenicity Morus/*genetics/;" |
| Notes: | "MedlineLi, Xiaotong Shi, Liangen Dai, Xiangping Chen, Yajie Xie, Hongqing Feng, Min Chen, Yuyin Wang, Huabing eng Research Support, Non-U.S. Gov't England 2018/05/13 Mol Ecol. 2018 Jul; 27(13):2858-2870. doi: 10.1111/mec.14720. Epub 2018 Jun 4" |
