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Talanta

Title:		Novel sampling strategy for alive animal volatolome extraction combined with GC-MS based untargeted metabolomics: Identifying mouse pup pheromones
Author(s):		Lacalle-Bergeron L; Goterris-Cerisuelo R; Portoles T; Beltran J; Sancho JV; Navarro-Moreno C; Martinez-Garcia F;
Address:		"Enviromental and Public Health Analytical Chemistry, Research Institute for Pesticides and Water (IUPA), Universitat Jaume I, Av. Sos Baynat S/N, 12071, Castellon de la Plana, Spain. Laboratory of Functional Neuroanatomy (Unitat Mixta NeuroFun-UV-UJI), Predepartamental Unit of Medicine, Universitat Jaume I, Av. Sos Baynat S/N, 12071, Castellon de la Plana, Spain. Enviromental and Public Health Analytical Chemistry, Research Institute for Pesticides and Water (IUPA), Universitat Jaume I, Av. Sos Baynat S/N, 12071, Castellon de la Plana, Spain. Electronic address: tportole@uji.es"
Journal Title:		Talanta
Year:		2021
Volume:		20210804
Issue:
Page Number:		122786 -
DOI:		10.1016/j.talanta.2021.122786
ISSN/ISBN:		1873-3573 (Electronic) 0039-9140 (Linking)
Abstract:		"In this study, we identify 11 mouse pup volatiles putatively involved in maternal care induction in adult females. For this purpose, we have adapted the dynamic headspace methodology to extract the volatolome of whole alive animals. Untargeted metabolomic methodology was used to compare the volatolome of neonatal (4-6 days) with elder pups until the age of weaning (21-23 days old). Pup volatolome was analyzed by gas chromatography (GC) coupled to single quadrupole mass spectrometry (MS) using automated thermal desorption for sample introduction. After data processing and multivariate statistical analysis, comparison with NIST spectral library allowed identifying compounds secreted preferentially by neonatal pups: di(propylen glycol) methyl ether, 4-nonenal, di(ethylene glycol) monobutyl ether, 2-phenoxyethanol, isomethyl ionone, tridecanal, 1,3-diethylbenzene, 1,2,4,5-tetramethylbenzene, 2-ethyl-p-xylene and tri(propylene glycol) methyl ether. Palmitic acid was enriched in the volatolome of fourth week youngsters compared to neonatal pups. The results demonstrated the great potential of the new sampling procedure combined with GC-MS based untargeted volatolomics to identify volatile pheromones in mammals"
Keywords:		Animals Gas Chromatography-Mass Spectrometry Mass Spectrometry Metabolomics Mice Pheromones *Volatile Organic Compounds/analysis Dynamic headspace Gc-ms Maternal care Mouse pheromones Untargeted metabolomics Volatolomics;
Notes:		"MedlineLacalle-Bergeron, Leticia Goterris-Cerisuelo, Rafael Portoles, Tania Beltran, Joaquin Sancho, Juan Vicente Navarro-Moreno, Cinta Martinez-Garcia, Fernando eng Netherlands 2021/09/15 Talanta. 2021 Dec 1; 235:122786. doi: 10.1016/j.talanta.2021.122786. Epub 2021 Aug 4"