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Biomolecules

Title:		"Isolation, Identification, and Bioinformatic Analysis of Antibacterial Proteins and Peptides from Immunized Hemolymph of Red Palm Weevil Rhynchophorus ferrugineus"
Author(s):		Knutelski S; Awad M; Lukasz N; Bukowski M; Smialek J; Suder P; Dubin G; Mak P;
Address:		"Department of Entomology, Institute of Zoology and Biomedical Research, Jagiellonian University, Gronostajowa 9 St., 30-387 Krakow, Poland. Department of Economic Entomology and Pesticides, Faculty of Agriculture, Cairo University, Giza 12613, Egypt. Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7 St., 30-387 Krakow, Poland. Department of Analytical Chemistry and Biochemistry, Faculty of Materials Sciences and Ceramics, AGH University of Science and Technology, Mickiewicza 30 Ave., 30-059 Krakow, Poland. Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7a, 30-387, Krakow, Poland"
Journal Title:		Biomolecules
Year:		2021
Volume:		20210111
Issue:		1
Page Number:		-
DOI:		10.3390/biom11010083
ISSN/ISBN:		2218-273X (Electronic) 2218-273X (Linking)
Abstract:		"Red palm weevil (Rhynchophorus ferrugineus Olivier, 1791, Coleoptera: Curculionidae) is a destructive pest of palms, rapidly extending its native geographical range and causing large economic losses worldwide. The present work describes isolation, identification, and bioinformatic analysis of antibacterial proteins and peptides from the immunized hemolymph of this beetle. In total, 17 different bactericidal or bacteriostatic compounds were isolated via a series of high-pressure liquid chromatography steps, and their partial amino acid sequences were determined by N-terminal sequencing or by mass spectrometry. The bioinformatic analysis of the results facilitated identification and description of corresponding nucleotide coding sequences for each peptide and protein, based on the recently published R. ferrugineus transcriptome database. The identified compounds are represented by several well-known bactericidal factors: two peptides similar to defensins, one cecropin-A1-like peptide, and one attacin-B-like protein. Interestingly, we have also identified some unexpected compounds comprising five isoforms of pheromone-binding proteins as well as seven isoforms of odorant-binding proteins. The particular role of these factors in insect response to bacterial infection needs further investigation"
Keywords:		"Amino Acid Sequence Animals Anti-Bacterial Agents/*isolation & purification Araceae/*parasitology Chromatography, High Pressure Liquid Chromatography, Reverse-Phase *Computational Biology Hemolymph/*immunology *Immunization Insect Proteins/chemistry/*isol;"
Notes:		"MedlineKnutelski, Stanislaw Awad, Mona Lukasz, Natalia Bukowski, Michal Smialek, Justyna Suder, Piotr Dubin, Grzegorz Mak, Pawel eng 2018/31/B/NZ3/01226/National Science Centre, Poland/ N18 DBS/000016/Jagiellonian University/ Research Support, Non-U.S. Gov't Switzerland 2021/01/15 Biomolecules. 2021 Jan 11; 11(1):83. doi: 10.3390/biom11010083"