| Title: | A new method for the determination of primary and secondary terrestrial and marine biomarkers in ice cores using liquid chromatography high-resolution mass spectrometry |
| Author(s): | King ACF; Giorio C; Wolff E; Thomas E; Karroca O; Roverso M; Schwikowski M; Tapparo A; Gambaro A; Kalberer M; |
| Address: | "British Antarctic Survey, High Cross, Madingley Road, Cambridge CB3 0ET, United Kingdom; Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom. Electronic address: acfk2@cam.ac.uk. Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom. Electronic address: chiara.giorio@unipd.it. Department of Earth Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EQ, United Kingdom. Electronic address: ew428@cam.ac.uk. British Antarctic Survey, High Cross, Madingley Road, Cambridge CB3 0ET, United Kingdom. Electronic address: lith@bas.ac.uk. Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom; Ca' Foscari University of Venice, Venice, Italy. Electronic address: ornela86@live.it. Dipartimento di Scienze Chimiche, Universita degli Studi di Padova, Via Marzolo 1, Padova 35131, Italy. Electronic address: marco.roverso@unipd.it. Paul Scherrer Institut, Forschungsstrasse 111, Villigen PSI 5232, Switzerland. Electronic address: margit.schwikowski@psi.ch. Dipartimento di Scienze Chimiche, Universita degli Studi di Padova, Via Marzolo 1, Padova 35131, Italy. Electronic address: andrea.tapparo@unipd.it. Ca' Foscari University of Venice, Venice, Italy. Electronic address: gambaro@unive.it. Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom. Electronic address: mk594@cam.ac.uk" |
| DOI: | 10.1016/j.talanta.2018.10.042 |
| ISSN/ISBN: | 1873-3573 (Electronic) 0039-9140 (Linking) |
| Abstract: | "The majority of atmospheric compounds measured in ice cores are inorganic, while analysis of their organic counterparts is a less well developed field. In recent years, understanding of formation, transport pathways and preservation of these compounds in ice and snow has improved, showing great potential for their use as biomarkers in ice cores. This study presents an optimised analytical technique for quantification of terrestrial and marine biosphere emissions of secondary organic aerosol (SOA) components and fatty acids in ice using HPLC-MS analysis. Concentrations of organic compounds in snow and ice are extremely low (typically ppb or ppt levels) and thus pre-concentration is required prior to analysis. Stir bar sorptive extraction (SBSE) showed potential for fatty acid compounds, but failed to recover SOA compounds. Solid phase extraction (SPE) recovered compounds across both organic groups but methods improving some recoveries came at the expense of others, and background contamination of fatty acids was high. Rotary evaporation was by far the best performing method across both SOA and fatty acid compounds, with average recoveries of 80%. The optimised preconcentration - HPLC-MS method achieved repeatability of 9% averaged for all compounds. In environmental samples, both concentrations and seasonal trends were observed to be reproducible when analysed in two different laboratories using the same method" |
| Keywords: | "Aerosols/*analysis/isolation & purification Aquatic Organisms/*chemistry Biomarkers/analysis Calibration Chromatography, Liquid/*methods Fatty Acids/*analysis/isolation & purification *Ice Reproducibility of Results Solid Phase Extraction Tandem Mass Spec;" |
| Notes: | "MedlineKing, Amy C F Giorio, Chiara Wolff, Eric Thomas, Elizabeth Karroca, Ornela Roverso, Marco Schwikowski, Margit Tapparo, Andrea Gambaro, Andrea Kalberer, Markus eng Netherlands 2019/01/06 Talanta. 2019 Mar 1; 194:233-242. doi: 10.1016/j.talanta.2018.10.042. Epub 2018 Oct 16" |
