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Pharm Biol

Title:		LC-MS-MS and GC-MS analyses of biologically active extracts and fractions from Tunisian Juniperus phoenice leaves
Author(s):		Keskes H; Belhadj S; Jlail L; El Feki A; Damak M; Sayadi S; Allouche N;
Address:		"a Laboratory of Chemistry of Natural Substances, Faculty of Sciences of Sfax , University of Sfax , Sfax , Tunisia. b Laboratory of Animal Ecophysiology, Faculty of Sciences of Sfax , University of Sfax , Sfax , Tunisia. c Laboratory of Environmental Bioprocesses , Center of Biotechnology of Sfax , Sfax , Tunisia"
Journal Title:		Pharm Biol
Year:		2017
Volume:		20160922
Issue:		1
Page Number:		88 - 95
DOI:		10.1080/13880209.2016.1230139
ISSN/ISBN:		1744-5116 (Electronic) 1388-0209 (Print) 1388-0209 (Linking)
Abstract:		"CONTEXT: Despite some studies related to Juniperus phoenicea L. (Cupressaceae), phytochemical and biological investigations of this plant remain unexplored. OBJECTIVE: This work is the first report dealing with the identification and characterization of volatile components and flavonoids in hexane and methanol extracts from J. phoenicea leaves Materials and methods: Antioxidant activity of hexane, and methanol extracts from J. phoenicea leaves were determined by DPPH-radical scavenging assay. alpha-Amylase inhibitory activity was evaluated by enzyme inhibition using in vitro assay (each extract was dissolved in DMSO to give concentrations of 50, 100 and 200 mg/mL). The chemical composition of fractions (Fr1-Fr3) from methanol extract was determined by high-performance liquid chromatography coupled with mass spectroscopy (HPLC-MS) analysis. RESULTS AND DISCUSSION: The hexane extract was analyzed by GC-MS technique which allowed the identification of 32 compounds. The main constituents were alpha-humulene (16.9%), pentadecane (10.2%) and alpha-cubebene (9.7%). Fraction Fr 2 exhibited a strong DPPH radical-scavenging activity (IC(50 )=( )20.1 mug/mL) compared to that of BHT as well as the highest alpha-amylase inhibitory activity (IC(50 )=( )28.4 mug/mL). Three flavonoids were identified in these fractions using HPLC-MS analysis: Quercetin 3-O-glucoside, isoscutellarein 7-O-pentoside and quercetin 3-O-pentoside. In addition, the more active fraction (Fr 2) was purified with semi-preparative HPLC affording one pure compound (amentoflavone) using (1)H NMR analysis. This compound exhibited powerful DPPH radical-scavenging (IC(50 )=( )14.1 mug/mL) and alpha-amylase inhibition (IC(50 )=( )20.4 mug/mL) effects. CONCLUSION: This study provides scientific support to some medicinal uses of J. phoenicea found in North Africa"
Keywords:		"Antioxidants/isolation & purification/pharmacology Biphenyl Compounds/chemistry *Chromatography, High Pressure Liquid Enzyme Inhibitors/isolation & purification/pharmacology Flavonoids/*isolation & purification/pharmacology *Gas Chromatography-Mass Spectr;"
Notes:		"MedlineKeskes, Henda Belhadj, Sahla Jlail, Lobna El Feki, Abdelfattah Damak, Mohamed Sayadi, Sami Allouche, Noureddine eng England 2016/12/08 Pharm Biol. 2017 Dec; 55(1):88-95. doi: 10.1080/13880209.2016.1230139. Epub 2016 Sep 22"