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J Bacteriol

Title:		Mapping of the Cryptococcus neoformans MATalpha locus: presence of mating type-specific mitogen-activated protein kinase cascade homologs
Author(s):		Karos M; Chang YC; McClelland CM; Clarke DL; Fu J; Wickes BL; Kwon-Chung KJ;
Address:		"Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA"
Journal Title:		J Bacteriol
Year:		2000
Volume:		182
Issue:		21
Page Number:		6222 - 6227
DOI:		10.1128/JB.182.21.6222-6227.2000
ISSN/ISBN:		0021-9193 (Print) 1098-5530 (Electronic) 0021-9193 (Linking)
Abstract:		"In this study we investigated the relationship between the MATalpha locus of Cryptococcus neoformans and several MATalpha-specific mitogen-activated protein (MAP) kinase signal transduction cascade genes, including STE12alpha, STE11alpha, and STE20alpha. To resolve the location of the genes, we screened a cosmid library of the MATalpha strain B-4500 (JEC21), which was chosen for the C. neoformans genome project. We isolated several overlapping cosmids spanning a region of about 71 kb covering the entire MATalpha locus. It was found that STE12alpha, STE11alpha, and STE20alpha are imbedded within the locus rather than closely linked to the locus. Furthermore, three copies of MFalpha, the mating type alpha-pheromone gene, a MATalpha-specific myosin gene, and a pheromone receptor (CPRalpha) were identified within the locus. We created a physical map, based on the restriction enzyme BamHI, and identified both borders of the MATalpha locus. The MATalpha locus of C. neoformans is approximately 50 kb in size and is one of the largest mating type loci reported among fungi with a one-locus, two-allele mating system"
Keywords:		"Chemoreceptor Cells Cosmids Cryptococcus neoformans/*genetics *Genes, Fungal *Genes, Mating Type, Fungal Mitogen-Activated Protein Kinases/*genetics Myosins/genetics Pheromones/genetics Receptors, Cell Surface Restriction Mapping;"
Notes:		"MedlineKaros, M Chang, Y C McClelland, C M Clarke, D L Fu, J Wickes, B L Kwon-Chung, K J eng Wellcome Trust/United Kingdom R29 AI043522/AI/NIAID NIH HHS/ R29AI43522/AI/NIAID NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 2000/10/13 J Bacteriol. 2000 Nov; 182(21):6222-7. doi: 10.1128/JB.182.21.6222-6227.2000"