| Title: | "A novel gene, msa1, inhibits sexual differentiation in Schizosaccharomyces pombe" |
| Author(s): | Jeong HT; Ozoe F; Tanaka K; Nakagawa T; Matsuda H; Kawamukai M; |
| Address: | "Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, Matsue 690-8504, Japan" |
| DOI: | 10.1534/genetics.167.1.77 |
| ISSN/ISBN: | 0016-6731 (Print) 0016-6731 (Linking) |
| Abstract: | "Sexual differentiation in the fission yeast Schizosaccharomyces pombe is triggered by nutrient starvation or by the presence of mating pheromones. We identified a novel gene, msa1, which encodes a 533-aa putative RNA-binding protein that inhibits sexual differentiation. Disruption of the msa1 gene caused cells to hypersporulate. Intracellular levels of msa1 RNA and Msa1 protein diminished after several hours of nitrogen starvation. Genetic analysis suggested that the function of msa1 is independent of the cAMP pathway and stress-responsive pathway. Deletion of the ras1 gene in diploid cells inhibited sporulation and in haploid cells decreased expression of mating-pheromone-induced genes such as mei2, mam2, ste11, and rep1; simultaneous deletion of msa1 reversed both phenotypes. Overexpression of msa1 decreased activated Ras1(Val17)-induced expression of mam2. Phenotypic hypersporulation was similar between cells with deletion of only rad24 and both msa1 and rad24, but simultaneous deletion of msa1 and msa2/nrd1 additively increased hypersporulation. Therefore, we suggest that the primary function of Msa1 is to negatively regulate sexual differentiation by controlling the expression of Ste11-regulated genes, possibly through the pheromone-signaling pathway" |
| Keywords: | "Amino Acid Sequence Base Sequence Blotting, Northern Blotting, Western Cell Cycle Proteins/physiology Cyclic AMP/metabolism Epitopes Gene Deletion Gene Library *Genes, Fungal Genotype Haploidy Intracellular Signaling Peptides and Proteins Meiosis Models, ;" |
| Notes: | "MedlineJeong, Hee Tae Ozoe, Fumiyo Tanaka, Katsunori Nakagawa, Tsuyoshi Matsuda, Hideyuki Kawamukai, Makoto eng Research Support, Non-U.S. Gov't 2004/05/29 Genetics. 2004 May; 167(1):77-91. doi: 10.1534/genetics.167.1.77" |
