| Title: | Biocatalytic Potential of Native Basidiomycetes from Colombia for Flavour/Aroma Production |
| Author(s): | Jaramillo DA; Mendez MJ; Vargas G; Stashenko EE; Vasco-Palacios AM; Ceballos A; Caicedo NH; |
| Address: | "Department of Biochemical Engineering, Universidad Icesi, Calle 18 No. 122-135 Pance, Cali 760031, Colombia. Universidad Industrial de Santander. Chromatography and Mass Spectrometry Center, Calle 9 Carrera 27, Bucaramanga 680002, Colombia. Grupo de Microbiologia Ambiental-BioMicro, Escuela de Microbiologia, Universidad de Antioquia, UdeA, Calle 70 No. 52-21, Medellin 050010, Colombia" |
| DOI: | 10.3390/molecules25184344 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "Aromas and flavours can be produced from fungi by either de novo synthesis or biotransformation processes. Herein, the biocatalytic potential of seven basidiomycete species from Colombia fungal strains isolated as endophytes or basidioma was evaluated. Ganoderma webenarium, Ganoderma chocoense, and Ganoderma stipitatum were the most potent strains capable of decolourizing beta,beta-carotene as evidence of their potential as biocatalysts for de novo aroma synthesis. Since a species' biocatalytic potential cannot solely be determined via qualitative screening using beta,beta-carotene biotransformation processes, we focused on using alpha-pinene biotransformation with mycelium as a measure of catalytic potential. Here, two strains of Trametes elegans-namely, the endophytic (ET-06) and basidioma (EBB-046) strains-were screened. Herein, T. elegans is reported for the first time as a novel biocatalyst for the oxidation of alpha-pinene, with a product yield of 2.9 mg of cis-Verbenol per gram of dry weight mycelia used. The EBB-046 strain generated flavour compounds via the biotransformation of a Cape gooseberry medium and de novo synthesis in submerged cultures. Three aroma-producing compounds were identified via GC-MS-namely, methyl-3-methoxy-4H-pyran-4-one, hexahydro-3-(methylpropyl)-pyrrolo[1,2-a]pyrazine-1,4-dione, and hexahydro-3-(methylphenyl)-pyrrolo[1,2-a]pyrazine-1,4-dione" |
| Keywords: | Animals Basidiomycota/*metabolism *Biocatalysis Biotransformation Colombia Odorants/*analysis *Taste Volatile Organic Compounds/chemistry/metabolism aromas basidiomycetes bioprospection cis-verbenol alpha-Pinene; |
| Notes: | "MedlineJaramillo, David A Mendez, Maria J Vargas, Gabriela Stashenko, Elena E Vasco-Palacios, Aida-M Ceballos, Andres Caicedo, Nelson H eng CA0213270./Universidad ICESI/ Switzerland 2020/09/26 Molecules. 2020 Sep 22; 25(18):4344. doi: 10.3390/molecules25184344" |
