| Title: | Odorant-binding proteins from a primitive termite |
| Author(s): | Ishida Y; Chiang VP; Haverty MI; Leal WS; |
| Address: | "Department of Entomology University of California, Davis 95616, USA" |
| ISSN/ISBN: | 0098-0331 (Print) 0098-0331 (Linking) |
| Abstract: | "Hitherto, odorant-binding proteins (OBPs) have been identified from insects belonging to more highly evolved insect orders (Lepidoptera, Coleoptera, Diptera, Hymenoptera, and Hemiptera), whereas only chemosensory proteins have been identified from more primitive species, such as orthopteran and phasmid species. Here, we report for the first time the isolation and cloning of odorant-binding proteins from a primitive termite species, the dampwood termite. Zootermopsis nevadensis nevadensis (Isoptera: Termopsidae). A major antennae-specific protein was detected by native PAGE along with four other minor proteins, which were also absent in the extract from control tissues (hindlegs). Multiple cDNA cloning led to the full characterization of the major antennae-specific protein (ZnevOBP1) and to the identification of two other antennae-specific cDNAs, encoding putative odorant-binding proteins (ZnevOBP2 and ZnevOBP3). N-terminal amino acid sequencing of the minor antennal bands and cDNA cloning showed that olfaction in Z. n. nevadensis may involve multiple odorant-binding proteins. Database searches suggest that the OBPs from this primitive termite are homologues of the pheromone-binding proteins from scarab beetles and antennal-binding proteins from moths" |
| Keywords: | "Amino Acid Sequence Animals Cloning, Molecular DNA, Complementary/genetics Isoptera/*genetics/*metabolism Molecular Sequence Data Pheromones/metabolism Receptors, Odorant/*genetics/*metabolism Reverse Transcriptase Polymerase Chain Reaction Sequence Align;" |
| Notes: | "MedlineIshida, Yuko Chiang, Vicky P Haverty, Michael I Leal, Walter S eng Research Support, Non-U.S. Gov't 2002/11/27 J Chem Ecol. 2002 Sep; 28(9):1887-93. doi: 10.1023/a:1020537319805" |
