| Title: | The influence of incubation on the formation of volatile bacterial metabolites in mastitis milk |
| Author(s): | Hettinga KA; van Valenberg HJ; Lam TJ; van Hooijdonk AC; |
| Address: | "Dairy Science and Technology group, Wageningen University and Research Centre, 6700EV Wageningen, the Netherlands. kasper.hettinga@wur.nl" |
| ISSN/ISBN: | 1525-3198 (Electronic) 0022-0302 (Linking) |
| Abstract: | "The possibility to detect mastitis-causing pathogens based on their volatile metabolites was previously studied. In that study, the mastitis samples were incubated overnight. To minimize the total analysis time, no incubation, or a short incubation, would be preferred. We therefore investigated the effect of the incubation time on the formation of volatile metabolites in mastitis samples. A selection of 6 volatile metabolites with the highest impact on the prediction model for identifying the mastitis-causing pathogen, was compared at different incubation times between 0 and 24 h. Identification of the pathogens was not possible without incubation. The minimum incubation time for detection of most of the 6 metabolites was 4 to 8 h. Although a longer incubation time increased the differences between pathogens, after 8 h all metabolites could be detected and the pathogens could be differentiated. Eight hours was therefore selected as the optimal incubation time. This optimal incubation time was evaluated with a set of 25 mastitis samples, of which 88% were correctly classified after 8 h of incubation. The total analysis time for this method is therefore considerably shorter than current microbiological culturing" |
| Keywords: | "Animals Bacteria/*isolation & purification/*metabolism Cattle Colony Count, Microbial Escherichia coli/isolation & purification/metabolism Female Mastitis, Bovine/*microbiology Milk/chemistry/*microbiology Neural Networks, Computer Staphylococcus aureus/i;" |
| Notes: | "MedlineHettinga, K A van Valenberg, H J F Lam, T J G M van Hooijdonk, A C M eng 2009/09/19 J Dairy Sci. 2009 Oct; 92(10):4901-5. doi: 10.3168/jds.2008-1818" |
