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Biopolymers

Title:		"NMR investigation of cyclo7,10[C7,X9,C10,Nle12] analogues of the alpha-factor from Saccharomyces cerevisiae"
Author(s):		Gounarides JS; Xue CB; Becker JM; Naider F;
Address:		"Department of Chemistry, Hunter College, New York, New York"
Journal Title:		Biopolymers
Year:		1994
Volume:		34
Issue:		6
Page Number:		709 - 720
DOI:		10.1002/bip.360340604
ISSN/ISBN:		0006-3525 (Print) 0006-3525 (Linking)
Abstract:		"The cyclo7,10[Cys7,Cys10,Nle12], cyclo7,10[Cys7,D-Ala9,Cys10,Nle12], and cyclo7,10[Cys7,L-Ala9,Cys10,Nle12] analogues of the alpha-factor mating pheromone (WHWLQLKPGQPMY) of the yeast Saccharomyces cerevisiae were studied in DMSO/water (80:20) and aqueous solution by nmr spectroscopy. In addition, the cyclo7,10[Cys7,D-Val9,Cys10,Nle12]alpha-fa ctor was examined in DMSO/water. Nuclear Overhauser effect (NOE) and NH d delta/dT data indicate that the cyclo7,10[Cys7,D-Val9,Cys10,Nle12]alpha-fa ctor adopts a type II beta-turn in DMSO/water and that the cyclo7,10[Cys7,D-Ala9,Cys10,Nle12]- and cyclo7,10[Cys7,L-Ala9,Cys10,Nle12]alpha-fa ctor analogues adopt type II and type I/III beta-turns, respectively, in both DMSO/water and aqueous solutions. In aqueous solution, residues 8 and 9 of the cyclo7,10[Cys7,Nle12] alpha-factor appear to adopt at least two distinct conformations, one of these being identified as a type I/III beta-turn. In contrast, the cyclo7,10[Cys7,Cys10,Nle12] alpha-factor appears to adopt predominately a type II beta-turn in DMSO/water. Quantitative NOE measurements of the cyclo7,10[Cys7,Cys10,Nle12]-, cyclo7,10[Cys7,D-Val9,Cys10,Nle12]-, and cyclo7,10[Cys7,L-Ala9,Cys10,Nle12] alpha-factors in DMSO/water were used to derive three-dimensional structures of the cyclo7,10[Cys7,Pro8,X9,Cys10] portion of these analogues"
Keywords:		"Amino Acid Sequence Fungal Proteins/*chemistry Magnetic Resonance Spectroscopy/methods Mating Factor Molecular Sequence Data Peptides/*chemistry Peptides, Cyclic/chemistry Pheromones/chemistry Protein Conformation Protein Structure, Secondary Saccharomyce;"
Notes:		"MedlineGounarides, J S Xue, C B Becker, J M Naider, F eng 5G12RR03037-07/RR/NCRR NIH HHS/ GM22086/GM/NIGMS NIH HHS/ GM22087/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1994/06/01 Biopolymers. 1994 Jun; 34(6):709-20. doi: 10.1002/bip.360340604"