| Title: | Functional identification of AtTPS03 as (E)-beta-ocimene synthase: a monoterpene synthase catalyzing jasmonate- and wound-induced volatile formation in Arabidopsis thaliana |
| Author(s): | Faldt J; Arimura G; Gershenzon J; Takabayashi J; Bohlmann J; |
| Address: | "Biotechnology Laboratory, Department of Botany, University of British Columbia, 237-6174 University Boulevard, Vancouver, V6T 1Z3, B.C., Canada" |
| DOI: | 10.1007/s00425-002-0924-0 |
| ISSN/ISBN: | 0032-0935 (Print) 0032-0935 (Linking) |
| Abstract: | "(E)-beta-Ocimene is one of the most commonly found monoterpenes of the volatile blends that are emitted from leaves in response to damage by herbivores or mechanical wounding. (E)-beta-Ocimene is also a component of many floral scents. Airborne (E)-beta-ocimene emitted from plants can serve as a chemical cue for the attraction of parasitoids or predators of plant herbivores and also as an attractant for pollinating insects. Furthermore, exposure of plants to (E)-beta-ocimene can activate defense gene expression. In this paper, we describe cDNA cloning and functional characterization of a gene encoding a highly specialized (E)-beta-ocimene synthase, AtTPS03, from Arabidopsis thaliana (L.) Heynh. AtTPS03 was identified as a member of the large AtTPS gene family of putative terpene synthases. A cDNA for AtTPS03 was expressed in Escherichia coli and the enzyme function identified in vitro. The A. thaliana (E)-beta-ocimene synthase produces almost exclusively (E)-beta-ocimene (94%) with minor amounts of the related acyclic monoterpenes (Z)-beta-ocimene (4%) and myrcene (2%). Transcripts for AtTPS03 were up-regulated in leaves of Arabidopsis in response to mechanical wounding and treatment with jasmonic acid, concurrent with induced emission of (E)-beta-ocimene. AtTPS03 provides an important gene for probing plant-insect and possibly plant-plant interactions mediated by terpenoid volatiles" |
| Keywords: | "Acyclic Monoterpenes Alkenes/chemistry/metabolism Amino Acid Sequence Arabidopsis/*enzymology/genetics Arabidopsis Proteins/genetics/metabolism Cloning, Molecular Cyclopentanes/*metabolism DNA, Complementary/chemistry/genetics Gene Expression Regulation, ;" |
| Notes: | "MedlineFaldt, Jenny Arimura, Gen-ichiro Gershenzon, Jonathan Takabayashi, Junji Bohlmann, Jorg eng Research Support, Non-U.S. Gov't Germany 2003/03/08 Planta. 2003 Mar; 216(5):745-51. doi: 10.1007/s00425-002-0924-0. Epub 2002 Oct 25" |
