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Plant Mol Biol

Title:		Characteristic alatoid 'cineole cassette' monoterpene synthase present in Nicotiana noctiflora
Author(s):		Fahnrich A; Neumann M; Piechulla B;
Address:		"Institute for Biological Sciences, University of Rostock, Albert-Einstein-Str. 3, 18059, Rostock, Germany"
Journal Title:		Plant Mol Biol
Year:		2014
Volume:		20140204
Issue:		1-Feb
Page Number:		135 - 145
DOI:		10.1007/s11103-014-0176-y
ISSN/ISBN:		1573-5028 (Electronic) 0167-4412 (Linking)
Abstract:		"Nicotiana species of the section Alatae emit a characteristic floral scent comprising the' cineole cassette' monoterpenes 1,8-cineole, limonene, myrcene, beta-pinene, alpha-pinene, sabinene and alpha-terpineol. All previously isolated 'cineole cassette'-monoterpene synthase genes are multi product enzymes that synthesize the seven compounds of the 'cineole cassette'. Interestingly, so far this 'alatoid' trait was only shared with the eponymous species Nicotiana suaveolens of the sister section Suaveolentes. To determine the origin of the 'cineole cassette' monoterpene phenotype other potential parent species of section Noctiflorae or Petunoides as well as of the distantly related section Trigonophyllae were analysed. A monoterpene synthase producing the set of 'cineole cassette' compounds was isolated from N. noctiflorae. N. obtusifolia emitted solely 1,8-cineole and no monoterpenes were found in floral scents of N. petunoides and N. palmeri. Interestingly, the phylogenetic analysis clustered the new gene of N. noctiflora closely to the terpineol synthase genes of e.g. N. alata rather than to cineole synthase genes of e.g. N. forgetiana"
Keywords:		"Amino Acid Sequence Cyclohexanols/metabolism Eucalyptol Intramolecular Lyases/chemistry/*metabolism Molecular Sequence Data Monoterpenes/metabolism Phylogeny Sequence Homology, Amino Acid Tobacco/classification/*enzymology Volatilization;"
Notes:		"MedlineFahnrich, Anke Neumann, Madeleine Piechulla, Birgit eng Research Support, Non-U.S. Gov't Netherlands 2014/02/05 Plant Mol Biol. 2014 May; 85(1-2):135-45. doi: 10.1007/s11103-014-0176-y. Epub 2014 Feb 4"