| Title: | Differential Volatile Organic Compound Expression in the Interaction of Daldinia eschscholtzii and Mycena citricolor |
| Author(s): | Escudero-Leyva E; Quiros-Guerrero L; Vasquez-Chaves V; Pereira-Reyes R; Chaverri P; Tamayo-Castillo G; |
| Address: | "Centro de Investigaciones en Productos Naturales (CIPRONA), Universidad de Costa Rica, 11520-2060 San Jose, Costa Rica. Escuela de Biologia, Universidad de Costa Rica, 11520-2060 San Jose, Costa Rica. Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, 1205 Geneva, Switzerland. School of Pharmaceutical Sciences, University of Geneva, 1205 Geneva, Switzerland. Laboratorio Nacional de Nanotecnologia (LANOTEC), Centro Nacional de Alta Tecnologia, 10109 San Jose, Costa Rica. Department of Natural Sciences, Bowie State University, Bowie, Maryland 20715, United States. Escuela de Quimica, Universidad de Costa Rica, 11520-2060 San Jose, Costa Rica" |
| ISSN/ISBN: | 2470-1343 (Electronic) 2470-1343 (Linking) |
| Abstract: | "Fungi exhibit a wide range of ecological guilds, but those that live within the inner tissues of plants (also known as endophytes) are particularly relevant due to the benefits they sometimes provide to their hosts, such as herbivory deterrence, disease protection, and growth promotion. Recently, endophytes have gained interest as potential biocontrol agents against crop pathogens, for example, coffee plants (Coffea arabica). Published results from research performed in our laboratory showed that endophytic fungi isolated from wild Rubiaceae plants were effective in reducing the effects of the American leaf spot of coffee (Mycena citricolor). One of these isolates (GU11N) from the plant Randia grandifolia was identified as Daldinia eschscholtzii (Xylariales). Its antagonism mechanisms, effects, and chemistry against M. citricolor were investigated by analyzing its volatile profile alone and in the presence of the pathogen in contactless and dual culture assays. The experimental design involved direct sampling of agar plugs in vials for headspace (HS) and headspace solid-phase microextraction (HS-SPME) gas chromatography-mass spectrometry (GC-MS) analysis. Additionally, we used ultrahigh-performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS/MS) to identify nonvolatile compounds from organic extracts of the mycelia involved in the interaction. Results showed that more volatile compounds were identified using HS-SPME (39 components) than those by the HS technique (13 components), sharing only 12 compounds. Statistical tests suggest that D. eschscholtzii inhibited the growth of M. citricolor through the release of VOCs containing a combination of 1,8-dimethoxynapththalene and terpene compounds affecting M. citricolor pseudopilei. The damaging effects of 1,8-dimethoxynaphthalene were corroborated in an in vitro test against M. citricolor pseudopilei; scanning electron microscopy (SEM) photographs confirmed structural damage. After analyzing the UHPLC-HRMS/MS data, a predominance of fatty acid derivatives was found among the putatively identified compounds. However, a considerable proportion of features (37.3%) remained unannotated. In conclusion, our study suggests that D. eschscholtzii has potential as a biocontrol agent against M. citricolor and that 1,8-dimethoxynaphthalene contributes to the observed damage to the pathogen's reproductive structures" |
| Notes: | "PubMed-not-MEDLINEEscudero-Leyva, Efrain Quiros-Guerrero, Luis Vasquez-Chaves, Victor Pereira-Reyes, Reinaldo Chaverri, Priscila Tamayo-Castillo, Giselle eng 2023/09/04 ACS Omega. 2023 Aug 18; 8(34):31373-31388. doi: 10.1021/acsomega.3c03865. eCollection 2023 Aug 29" |
