| Title: | Overexpression of TAT-PTD-diapause hormone fusion protein in tobacco and its effect on the larval development of Helicoverpa armigera (Lepidoptera: Noctuidae) |
| Author(s): | Zhou Z; Li Y; Yuan C; Doucet D; Zhang Y; Qu L; |
| Address: | "College of Forestry, Henan University of Science and Technology, Luoyang, China. Great Lakes Forestry Centre, Canadian Forest Service, Natural Resources Canada, Sault Sainte Marie, ON, Canada. Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing, China" |
| ISSN/ISBN: | 1526-4998 (Electronic) 1526-498X (Linking) |
| Abstract: | "BACKGROUND: The diapause hormone (DH) has been shown either to induce or to terminate diapause, depending on the insect species. In a previous study we demonstrated that the DH from Clostera anastomosis (caDH) has biological activity in Helicoverpa armigera, which prompted us to examine the potential growth-inhibiting or antiherbivory effects of the TAT-caDH fusion protein when expressed in transgenic plants. RESULTS: In this study, we produced transgenic tobacco plants expressing either the TAT-caDH protein or a TAT-caDH-eGFP fusion version that allowed tracking of the fluorescent protein in plant tissues. Our results indicate that H. armigera larvae feeding on transgenic tobacco expressing TAT-caDH exhibited a significantly reduced survival rate and weight gain. However, larvae feeding on transgenic tobacco expressing TAT-caDH-eGFP were unaffected. While fusion of the eGFP gene influenced the bioactivity of caDH in larvae, TAT-caDH-eGFP can still penetrate the insect midgut cell membrane. CONCLUSION: TAT-caDH increases DH stability in oral delivery. Our results may help in targeting DH-dependent physiological processes in insects for improving herbivore tolerance in economically important crops. (c) 2016 Society of Chemical Industry" |
| Keywords: | "Animals Herbivory Insect Hormones/genetics/*metabolism Insect Proteins/*genetics/metabolism Larva/genetics/growth & development/physiology Moths/genetics/growth & development/*physiology Neuropeptides/*genetics/metabolism Plants, Genetically Modified/gene;" |
| Notes: | "MedlineZhou, Zhou Li, Yongli Yuan, Chunyan Doucet, Daniel Zhang, Yongan Qu, Liangjian eng England 2016/09/20 Pest Manag Sci. 2017 Jun; 73(6):1197-1203. doi: 10.1002/ps.4443. Epub 2016 Nov 24" |
