Title: | "A sex pheromone, protoplast release-inducing protein (PR-IP) inducer, induces sexual cell division and production of PR-IP in Closterium" |
Author(s): | Tsuchikane Y; Fujii T; Ito M; Sekimoto H; |
Address: | "Department of General Systems Studies, Graduate School of Arts and Sciences, University of Tokyo, 3-8-1 Komaba, Meguro, Tokyo, 153-8902 Japan. tsuchik@dolphin.c.u-tokyo.ac.jp" |
ISSN/ISBN: | 0032-0781 (Print) 0032-0781 (Linking) |
Abstract: | "The sex pheromone protoplast release-inducing protein (PR-IP) inducer and a sexual cell division-inducing pheromone-minus (SCD-IP-minus) that mediates the sexual reproduction of the heterothallic Closterium peracerosum-strigosum-littorale (C. psl) complex were investigated in this study. Recombinant PR-IP inducer produced by yeast cells was prepared and assayed for production of PR-IP and induction of SCD. Both biological activities were observed after treating mating-type plus (mt+) cells with the recombinant pheromone. SCD was induced by exposure to a lower concentration of the same pheromone and by a shorter treatment period with the pheromone than was production of PR-IP. This indicates that the previously characterized PR-IP inducer has both PR-IP-inducing and SCD-inducing activities with mt+ cells, although the inducing mechanisms of the two pheromones differ" |
Keywords: | "Cell Division/*physiology Electrophoresis, Polyacrylamide Gel Eukaryota/cytology/*metabolism Plant Proteins/biosynthesis/*physiology Sex Attractants/*physiology;" |
Notes: | "MedlineTsuchikane, Yuki Fujii, Tadashi Ito, Motomi Sekimoto, Hiroyuki eng Research Support, Non-U.S. Gov't Japan 2005/06/23 Plant Cell Physiol. 2005 Sep; 46(9):1472-6. doi: 10.1093/pcp/pci158. Epub 2005 Jun 20" |