| Title: | Both farnesyl diphosphate synthase genes are involved in the production of alarm pheromone in the green peach aphid Myzus persicae |
| Address: | "Department of Entomology and MOA Key Laboratory for Monitoring and Environment-Friendly Control of Crop Pests, College of Plant Protection, China Agricultural University, Beijing, China" |
| Journal Title: | Arch Insect Biochem Physiol |
| ISSN/ISBN: | 1520-6327 (Electronic) 0739-4462 (Linking) |
| Abstract: | "Farnesyl diphosphate synthase (FPPS) catalyzes the formation of FPP, providing the precursor for the biosynthesis of (E)-beta-farnesene (EbetaF) in plants, but it is unknown if FPPS supplies the precursor for the biosynthesis of EbetaF, the major component of aphid alarm pheromone, though our previous studies support the hypothesis that EbetaF is synthesized by the aphid itself. Here, we used two cohorts of the green peach aphid Myzus persicae separately, reared on pepper plant and artificial diet to test the correlations among droplet emission, EbetaF quantity, and FPPS gene expression. It was found that the proportion of aphids emitting cornicle droplets and the quantity of EbetaF per milligram of aphid were both significantly different between the two cohorts, which were positively correlated with the expression of the two FPPS genes ( MpFPPS1/ 2) in M. persicae. These results were further confirmed by RNAi-mediated knockdown of MpFPPS1/ 2. Specifically, knockdown of MpFPPS1/ 2 imposed no significant cost on the survival of aphid but remarkably increased the number of offspring per aphid; most importantly, knockdown of MpFPPS1/ 2 significantly reduced the proportion of aphids emitting droplets and the quantity of EbetaF calculated as per the weight of aphid. Our results suggest that both FPPS genes are involved in the production of EbetaF in M. persicae and cornicle droplet emission is closely associated with the EbetaF release in the aphid" |
| Keywords: | Animals Aphids/enzymology/*genetics/growth & development/metabolism Geranyltranstransferase/*genetics/metabolism Insect Proteins/*genetics/metabolism Nymph/enzymology/genetics/growth & development/metabolism Pheromones/*biosynthesis (E)-beta-farnesene Myz; |
| Notes: | "MedlineCheng, Yin-Jie Li, Zheng-Xi eng 31772169/National Natural Science Foundation of China/ 31371940/National Natural Science Foundation of China/ 2018/12/21 Arch Insect Biochem Physiol. 2019 Mar; 100(3):e21530. doi: 10.1002/arch.21530. Epub 2018 Dec 19" |
