« Previous Abstract"Quantitative variation and biosynthesis of hindgut volatiles associated with the red turpentine beetle, Dendroctonus valens LeConte, at different attack phases"    Next Abstract"Syntheses of racemic and diastereomeric mixtures of 3,7,11,15-tetramethylhentriacontane and 4,8,12,16-tetramethyldotriacontane, the cuticular tetramethylalkanes of the tsetse fly, Glossina brevipalpis" »

Genome Biol Evol

Title:		Transcriptomes of mouse olfactory epithelium reveal sexual differences in odorant detection
Author(s):		Shiao MS; Chang AY; Liao BY; Ching YH; Lu MY; Chen SM; Li WH;
Address:		"Genomics Research Center, Academia Sinica, Taipei, Taiwan, ROC"
Journal Title:		Genome Biol Evol
Year:		2012
Volume:		20120417
Issue:		5
Page Number:		703 - 712
DOI:		10.1093/gbe/evs039
ISSN/ISBN:		1759-6653 (Electronic) 1759-6653 (Linking)
Abstract:		"To sense numerous odorants and chemicals, animals have evolved a large number of olfactory receptor genes (Olfrs) in their genome. In particular, the house mouse has ~1,100 genes in the Olfr gene family. This makes the mouse a good model organism to study Olfr genes and olfaction-related genes. To date, whether male and female mice possess the same ability in detecting environmental odorants is still unknown. Using the next generation sequencing technology (paired-end mRNA-seq), we detected 1,088 expressed Olfr genes in both male and female olfactory epithelium. We found that not only Olfr genes but also odorant-binding protein (Obp) genes have evolved rapidly in the mouse lineage. Interestingly, Olfr genes tend to express at a higher level in males than in females, whereas the Obp genes clustered on the X chromosome show the opposite trend. These observations may imply a more efficient odorant-transporting system in females, whereas a more active Olfr gene expressing system in males. In addition, we detected the expression of two genes encoding major urinary proteins, which have been proposed to bind and transport pheromones or act as pheromones in mouse urine. This observation suggests a role of main olfactory system (MOS) in pheromone detection, contrary to the view that only accessory olfactory system (AOS) is involved in pheromone detection. This study suggests the sexual differences in detecting environmental odorants in MOS and demonstrates that mRNA-seq provides a powerful tool for detecting genes with low expression levels and with high sequence similarities"
Keywords:		"Animals;Animals Evolution, Molecular Female Gene Expression Regulation High-Throughput Nucleotide Sequencing Male Mice *Olfactory Mucosa/metabolism *Pheromones/genetics/metabolism Receptors, Odorant/*genetics/*metabolism Sex Characteristics Transcriptome/genetics;"
Notes:		"MedlineShiao, Meng-Shin Chang, Andrew Ying-Fei Liao, Ben-Yang Ching, Yung-Hao Lu, Mei-Yeh Jade Chen, Stella Maris Li, Wen-Hsiung eng Research Support, Non-U.S. Gov't England 2012/04/19 Genome Biol Evol. 2012; 4(5):703-12. doi: 10.1093/gbe/evs039. Epub 2012 Apr 17"