| Title: | Tiered assembly of the yeast Far3-7-8-9-10-11 complex at the endoplasmic reticulum |
| Address: | "Department of Biological Sciences, University of New Orleans, New Orleans, Louisiana 70148. Department of Biological Sciences, University of New Orleans, New Orleans, Louisiana 70148. Electronic address: zliu5@uno.edu" |
| ISSN/ISBN: | 1083-351X (Electronic) 0021-9258 (Print) 0021-9258 (Linking) |
| Abstract: | "Target of rapamycin signaling is a conserved, essential pathway integrating nutritional cues with cell growth and proliferation. The target of rapamycin kinase exists in two distinct complexes, TORC1 and TORC2. It has been reported that protein phosphatase 2A (PP2A) and the Far3-7-8-9-10-11 complex (Far complex) negatively regulate TORC2 signaling in yeast. The Far complex, originally identified as factors required for pheromone-induced cell cycle arrest, and PP2A form the yeast counterpart of the STRIPAK complex, which was first isolated in mammals. The cellular localization of the Far complex has yet to be fully characterized. Here, we show that the Far complex localizes to the endoplasmic reticulum (ER) by analyzing functional GFP-tagged Far proteins in vivo. We found that Far9 and Far10, two homologous proteins each with a tail-anchor domain, localize to the ER in mutant cells lacking the other Far complex components. Far3, Far7, and Far8 form a subcomplex, which is recruited to the ER by Far9/10. The Far3-7-8- complex in turn recruits Far11 to the ER. Finally, we show that the tail-anchor domain of Far9 is required for its optimal function in TORC2 signaling. Our study reveals tiered assembly of the yeast Far complex at the ER and a function for Far complex's ER localization in TORC2 signaling" |
| Keywords: | "Cell Cycle Proteins/genetics/*metabolism Endoplasmic Reticulum/genetics/*metabolism Mechanistic Target of Rapamycin Complex 2 Multiprotein Complexes/genetics/*metabolism Mutation Protein Phosphatase 2/genetics/metabolism Protein Structure, Tertiary Saccha;" |
| Notes: | "MedlinePracheil, Tammy Liu, Zhengchang eng R15 GM094772/GM/NIGMS NIH HHS/ 1R15GM094772-01A1/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2013/04/30 J Biol Chem. 2013 Jun 7; 288(23):16986-16997. doi: 10.1074/jbc.M113.451674. Epub 2013 Apr 26" |
