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Genet Mol Res

Title:		"Genome-wide identification and expression profiling of the fatty acid desaturase gene family in the silkworm, Bombyx mori"
Author(s):		Chen QM; Cheng DJ; Liu SP; Ma ZG; Tan X; Zhao P;
Address:		"State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China zhaop@swu.edu.cn"
Journal Title:		Genet Mol Res
Year:		2014
Volume:		20140513
Issue:		2
Page Number:		3747 - 3760
DOI:		10.4238/2014.May.13.2
ISSN/ISBN:		1676-5680 (Electronic) 1676-5680 (Linking)
Abstract:		"Fatty acid desaturases exist in all living organisms and play important roles in many different biologic processes, such as fatty acid metabolism, lipid biosynthetic processes, and pheromone biosynthetic processes. Using the available silkworm genome sequence, we identified 14 candidate fatty acid desaturase genes. Eleven genes contain 3 conserved histidine cluster motifs and 4 transmembrane domains, but their N-terminal residues exhibit obvious diversity. Phylogenetic analysis revealed that there are 6 groups; Bmdesat1 and Bmdesat5-8 were clustered into group 2, which is involved in Delta11 desaturation activity, and Bmdesat3-4 were grouped in group 1, which is involved in Delta9 desaturation activity. Twelve of the 14 genes have expressed sequence tag evidence. Microarray data and reverse transcription polymerase chain reaction analysis demonstrated that Bmdesat3-4 and Bmdesat10 were expressed from the larval to moth stages and in multiple tissues on day 3 of 5th instar larvae. Bmdesat9, Bmdesat11, and Bmdesat14 were expressed during the pupal and late-embryonic stage, suggesting that they may take part in fatty acid metabolism to provide energy. These results provide some insights into the functions of individual fatty acid desaturases in silkworm"
Keywords:		"Amino Acid Sequence Animals Bombyx/*enzymology/genetics Cloning, Molecular Expressed Sequence Tags Fatty Acid Desaturases/biosynthesis/*genetics Fatty Acids/*metabolism Gene Expression Profiling Gene Expression Regulation, Enzymologic Genome, Insect Multi;"
Notes:		"MedlineChen, Q M Cheng, D J Liu, S P Ma, Z G Tan, X Zhao, P eng Research Support, Non-U.S. Gov't Brazil 2014/05/24 Genet Mol Res. 2014 May 13; 13(2):3747-60. doi: 10.4238/2014.May.13.2"