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Genes Dev

Title:		A protein involved in minichromosome maintenance in yeast binds a transcriptional enhancer conserved in eukaryotes
Author(s):		Passmore S; Elble R; Tye BK;
Address:		"Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853"
Journal Title:		Genes Dev
Year:		1989
Volume:		3
Issue:		7
Page Number:		921 - 935
DOI:		10.1101/gad.3.7.921
ISSN/ISBN:		0890-9369 (Print) 0890-9369 (Linking)
Abstract:		"The Saccharomyces cerevisiae MCM1 gene product is a protein with multiple functions. It is a transcription factor necessary for expression of mating-type-specific genes and is also required for the maintenance of minichromosomes. MCM1 shows DNA-binding specificities similar to those of two previously reported DNA-binding factors, pheromone/receptor transcription factor (PRTF) and general regulator of mating type (GRM); like PRTF, its activity can be modulated by the alpha 1 protein. MCM1 binds to the dyad symmetry element 5'-CCTAATTAGG and related sequences, which we refer to as MCM1 control elements (MCEs). MCEs are found within the regulatory regions of a- and alpha-specific genes. Direct and indirect DNA binding assays suggest that a conserved 5'-ATTAGG in one-half of the dyad symmetry element is important for MCM1 binding whereas variants in the other half are tolerated. We have used a novel DNase I 'nicking interference' assay to investigate the interaction of MCM1 with its substrate. These data suggest that MCM1 binds as a dimer, interacting symmetrically with the ATTAGG residues in each half of the binding site. MCM1 contains striking homology to the DNA-binding domain of the human serum response factor (SRF) which mediates the transient transcriptional activation of growth-stimulated genes by binding to the serum response element (SRE). We have shown that MCM1 binds to the human c-fos SRE in vitro and, like other MCEs, the c-fos SRE exhibits MCM1-mediated upstream activating sequence (UAS) activity in vivo"
Keywords:		"Base Sequence Binding, Competitive DNA, Fungal/*metabolism Deoxyribonucleases/metabolism *Enhancer Elements, Genetic Escherichia coli/genetics Molecular Sequence Data Nucleic Acid Hybridization Plasmids Promoter Regions, Genetic Protein Binding Regulatory;"
Notes:		"MedlinePassmore, S Elble, R Tye, B K eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. 1989/07/01 Genes Dev. 1989 Jul; 3(7):921-35. doi: 10.1101/gad.3.7.921"